摘要
[目的]exocyst是真核生物中普遍存在的囊泡拴系因子复合体。在模式植物拟南芥中,exocyst复合体参与多种重要的细胞生物学过程,如生长素运输、细胞极性生长、细胞板形成和细胞自噬等。探索exocyst复合体更多的生物学功能,对于了解exocyst复合体作用的分子机制具有重要意义。[方法]以exocyst复合体亚基SEC8基因花粉拯救的孢子体突变体PRsec8m1为材料,通过台盼蓝、DAPI、DHCF-DC等染料染色,结合遗传学试验分析其主根发育表型。[结果]发现exocyst复合体亚基SEC8基因突变导致主根细胞程序性死亡(PCD),阻断水杨酸信号传递途径不能缓解这种PCD表型。[结论]exocyst复合体亚基SEC8基因在主根发育过程中起关键作用,其突变导致主根细胞程序性死亡,这种细胞程序性死亡过程可能不依赖于水杨酸信号途径。
[Objectives]Exocyst is a vesicle tethering complex that exits in most eukaryotic organisms. In Arabidopsis thaliana,exocyst complex participates in many vital processes such as auxin polar transport,cell polar growth,cytokinesis,autophagy,etc. To understand the molecular roles of exocyst complex in detail,further exploration of its physiological functions is necessary. [Methods]In this study,an exocyst subunit SEC8 gene sporophytic mutant PRsec8m1 was generated by pollen rescued of sec8m1. Trypan blue staining,DHCF-DC staining,DAPI staining,TUNEL assay and genetic strategy were used to analyze the primary root growth defects in this mutant. [Results]We found that PRsec8m1 mutant primary roots underwent programmed cell death（ PCD）. Blocking salicylic acid（ SA） signal pathway could not rescue this phenotype. [Conclusions]Exocyst complex subunit SEC8 was essential for primary root development. Mutation in SEC8 gene led to PCD in primary root,which might be independent on SA signal pathway.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2016年第2期228-234,共7页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(31200236)
