摘要
目的确定丹参活血益脉丸中丹参酮ⅡA的提取方法,建立丹参活血益脉丸中丹参酮ⅡA的含量测定方法。方法 2014年12月采用不同提取溶剂、提取方法、提取时间确定丹参酮ⅡA的提取方法。采用高效液相色谱法测定丹参活血益脉丸中丹参酮ⅡA含量。色谱条件为色谱柱：Synergi Hydro-RP 80A（4.6×250 mm）;流动相：甲醇-水（75：25）,检测波长：270 nm,柱温：35℃。结果丹参活血益脉丸中丹参酮ⅡA的提取方法为甲醇回流提取1 h。丹参酮ⅡA在0.049 2~0.172 2μg范围内线性关系良好,平均回收率97.7%,RSD为1.41%。结论采用该方法提取丹参酮ⅡA结果准确,重现性好,专属性强,分离效果好,能有效控制丹参活血益脉丸的质量。
Objective Determine the method of extracting tanshinone ⅡAin compound Danshen Huoxue Yimai pills;establish the method of content determination of tanshinoneⅡAin compound Danshen Huoxue Yimai pills.Methods December 2014,using different extraction solvent,extraction method,extraction time to determine the extraction of tanshinone ⅡA.Determination the contents of tanshinone ⅡAin compound Danshen Huoxue Yimai pills by HPLC.The chromatographic conditions are column：Synergi Hydro-RP 80A（4.6×250 mm）; mobile phase： methanol-water（75：25）,detection wavelength：270 nm,column temperature：35 ℃.Results The extracting method of tanshinone ⅡAin compound Danshen Huoxue Yimai pills was methanol reflux 1 h.The content of Tanshinone ⅡAin the range of 0.049 2~0.172 2 μg has good linear relationship,average recovery was 97.7%,RSD was1.41%.Conclusion The method is accurate,reproducible,specific and good separation effect can effectively control the quality of the preparation.
出处
《社区医学杂志》
2016年第5期21-23,共3页
Journal Of Community Medicine
关键词
丹参活血益脉丸
含量测定
丹参酮ⅡA
高效液相色谱
Compound Danshen Huoxue Yimai pills
Content determination
TanshinoneⅡA
High performance liquid
