摘要
背景:盐酸川芎嗪具有促进神经修复的作用,可以应用于神经系统损伤的治疗。目的:观察盐酸川芎嗪与骨髓间充质干细胞移植联合应用大鼠脊髓损伤后的干预对电生理及后肢功能变化的影响。方法:采用Allen打击法建立T9脊髓全横断损伤模型大鼠,建模后随机分为3组,对照组仅尾静脉注射培养液;骨髓间充质干细胞移植组于尾静脉注射等体积的骨髓间充质干细胞;联合组尾静脉注射骨髓间充质干细胞+尾静脉滴注盐酸川芎嗪,持续4 h。造模后1,2,4,6,8周后进行BBB评分、改良Tarlov评分检测大鼠后肢运动功能恢复情况;于造模后72 h采用RT-PCR法检测各组大鼠脊髓损伤区周围Bcl-2、碱性成纤维细胞生长因子的表达变化;于造模后4周行体感诱发电位和运动诱发电位进行神经电生理恢复程度测定;造模后8周行辣根过氧化物酶示踪评估大鼠脊髓神经纤维的再生状况,通过PKH-26标记观察移植细胞的存活、迁移。结果与结论:造模后1,2,4,6,8周,联合组大鼠后肢BBB评分、改良Tarlov评分联合组明显优于骨髓间充质干细胞移植组,骨髓间充质干细胞移植组明显优于对照组(P<0.05),造模后72 h,PCR法检测结果表明联合组中大鼠脊髓损伤区周围Bcl-2、碱性成纤维细胞生长因子的表达显著高于骨髓间充质干细胞移植组及对照组(P<0.05)。造模后4周,各组体感和运动诱发电位的潜伏期:联合组<骨髓间充质干细胞移植组<对照组(P<0.05)。体感和运动诱发电位波幅宽度依次为联合组>骨髓间充质干细胞移植组>对照组(P<0.05)。造模后8周,骨髓间充质干细胞移植组及联合组有辣根过氧化物酶标记的神经锥体细胞明显的穿越痕迹;联合组中PKH-26标记的阳性细胞数与辣根过氧化物酶阳性细胞数量最多,骨髓间充质干细胞移植组次之,对照组相对最少(P<0.05)。研究表明,盐酸川芎嗪联合骨髓间充质干细胞移植可促进脊髓�
BACKGROUND： Ligustrazine hydrochloride which promotes nerve repair can be applied to the treatment of nervous system injury. OBJECTIVE： To investigate the effect of ligustrazine hydrochloride combined with bone marrow mesenchymal stem cells transplantation on electrophysiological property and hindlimb function of rats with spinal cord injury. METHODS： T9 spinal cord transection injury models were made in rats using Allen＇s method, and then rat models were randomized into three groups： rats in control group received tail vein injection of culture solution; rats in cell transplantation group underwent bone marrow mesenchymal stem cell transplantation via the tail vein; rats in combined group were subjected to the tail vein injection of ligustrazine hydrochloride and bone marrow mesenchymal stem cells that lasted for 4 hours. At 1, 2, 4, 6, 8 weeks after modeling, Basso, Beattie and Bresnahan scores and modified Tarlov scores were used to detect the motor function of rats. At 72 hours after modeling, RT-PCR method was used to detect the expression of Bcl-2 and basic fibroblast growth factor around the injured region. At 4 weeks after modeling, somatosensory and motor evoked potentials were measured for evaluation of neurophysiological recovery. At 8 weeks after modeling, horseradish peroxidase tracer was used to assess the regeneration of rat spinal cord nerve fibers; PKH-26 labeling was used to observe the survival and migration of transplanted cells. RESULTS AND CONCLUSION： At 1, 2, 4, 6, 8 weeks after modeling, Basso, Beattie and Bresnahan scores and modified Tarlov scores were significantly higher in the combined group than the cell transplantation followed by the control group（P〈0.05）. At 72 hours after modeling, the expression of Bcl-2 and basic fibroblast growth factor around the injured region was significantly higher in the combined group than the cell transplantation group and control group（P〈0.05）. At 4 weeks after modeling, the latencies of somatosensory and motor evoked potential
出处
《中国组织工程研究》
CAS
北大核心
2016年第1期95-101,共7页
Chinese Journal of Tissue Engineering Research
基金
河北大学医学学科专项基金建设项目(2012B2001)~~
