摘要
【目的】明确截形叶螨Tetranychus truncatus Ehara对哒螨灵、阿维菌素和阿维·哒螨灵3种田间常用药剂产生抗性的速率和稳定性,为叶螨的抗性综合治理提供一定的理论依据。【方法】采用室内生测法,对截形叶螨进行药剂的抗性筛选、衰退和再恢复规律研究。【结果】经过连续30代的药剂汰选,截形叶螨对哒螨灵、阿维菌素和阿维·哒螨灵3种药剂产生了不同程度的抗药性,抗性指数分别达到197.50、19.56和12.57;停止喷药后,其抗性都有所下降,其中截形叶螨对哒螨灵的抗性最不稳定,培育至30代后,抗性衰退率达到63.54%,对阿维菌素的抗性较为稳定,抗性衰退率为23.30%;再次恢复用药后,截形叶螨对哒螨灵、阿维菌素和阿维哒螨灵抗性再度回升,以抗哒螨灵品系的抗性恢复最快,药剂汰选30代后,增长率达到了58.47%,阿维·哒螨灵次之(增长率为38.67%),抗阿维菌素的品系抗性恢复最慢,增长率仅为22.86%。【结论】截形叶螨对哒螨灵抗性不稳定,停止用药后,敏感性易恢复,对阿维菌素和阿维·哒螨灵的抗性较稳定,一旦抗性产生不易衰退,故田间应用时应交替轮换用药。
[Objectives] To clarify the development of pesticide resistance, and the stability of resistance, to pyridaben, avermectin and avermectin＋pyridaben in Tetranychus truncatus Ehara. [Methods] Biological assays were carried out in a laboratory to detect selection for resistance to pyridaben, avermectin, and avermectin＋pyridaben, in T. truncatus, and the decline and recovery of resistance to these acaricides. [Results] After 30 generations of selection for resistance to the three acaricides, the resistance index to each increased by 197.50、19.56 and 12.57 folds, respectively. The resistance of a pyridaben resistant strain decreased very quickly in the absence of exposure to this acaricide, declining by 63.54% after 30 generations, whereas the resistance of avermectin resistant strain declined by 23.30% over the same number of generations. Resistance increased quickly after exposure to acaricides for about 30 generations. Resistance of the pyridaben resistant strain rapidly increased by 58.47%, but the recovery rate of the avermectin resistant strain was only 22.86%. The recovery rate of an abameetin＋pyridaben resistant strain was intermediate; 38.67%. [Conclusion] The resistance of T. truncatus to pyridaben was relatively unstable and sensitivity to this acaricide returned relatively quickly. Resistance to avermectin and abameetin＋ pyridaben were more stable, and sensitivity to these acaricides was difficult to reestablish. Consequently, avermectin should be used in rotation with the other insecticides to avoid cross-resistance and reduce selection pressure, thereby slowing the development of resistance in T. truncatus.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2016年第1期89-94,共6页
Chinese Journal of Applied Entomology
基金
公益性行业(农业)科研专项(201103020)
国家自然科学基金项目(31260442)
盛彤笙科技创新基金项目(GSAU-STS-1518)
关键词
截形叶螨
哒螨灵
阿维菌素
阿维·哒螨灵
抗药性
Tetranychus truncatus（Ehara）
pyridaben
avermectin
abameetin＋pyridaben
resistance
