摘要
目的:建立狗皮膏的气相色谱指纹图谱。方法:采用气相色谱法。色谱柱为弹性石英毛细管柱,程序升温,进样口温度为220℃,检测器为氢火焰离子化检测器,温度为250℃,分流进样量为1μl,分流比为20∶1。以丁香酚色谱峰为参照峰,分析5家生产企业32批狗皮膏,采用中药指纹图谱相似度计算软件(2009版)进行共有峰鉴定和相似度评价,并对32批样品的特征图谱进行聚类分析。结果:32批狗皮膏共有11个峰,5家生产企业32批样品间特征图谱相似度低,可聚为5类。结论:所建立的特征图谱专属性强,结果稳定,可为狗皮膏的质量评估和控制提供依据。
OBJECTIVE：To establish the GC specific chromatogram of Goupi cream. METHODS：Gas chromatography was performed on the column of fused silica capillary column by programmed temperature,injection temperature was 220 ℃,detector was hydrogen flame ionization detector with temperature of 250 ℃,the injection volume was 1 μl and split ratio of 20 ∶ 1. The eugenol chromatographic peak was used as reference peak to determine the 32 batches of Goupi cream from 5 enterprises,TCM fingerprint similarity calculation software（2009 edition） was conducted for the common peaks identification and similarity evaluation,and the specific chromatogram cluster analysis of 32 batches of samples. RESULTS：There were totally 11 common peaks in the 32 batches of Goupi cream,the 32 batches of samples from different enterprises showed low similarity and could be clustered into 5types. CONCLUSIONS：The established specific chromatogram is specific and stable,and can provide reference for the quality evaluation and control of Goupi cream.
出处
《中国药房》
CAS
北大核心
2016年第9期1264-1266,共3页
China Pharmacy
关键词
狗皮膏
气相色谱法
特征图谱
聚类分析
Goupi cream
Gas chromatography
Specific chromatogram
Cluster analysis
