摘要
目的:探索镇肝熄风汤对自发性高血压大鼠视网膜细胞的保护作用机制。方法:将48只SHR随机分为4组:模型组(B)、贝那普利对照组(C)、镇肝熄风汤低剂量观察组(D)、镇肝熄风汤高剂量观察组(E);每组各12只。正常空白组WKY(A)12只。镇肝熄风汤低剂量组灌服镇肝熄风汤15g·kg^(-1)·d^(-1)(药物浓度1.5g/m L);镇肝熄风汤高剂量组灌服镇肝熄风汤30g·kg^(-1)·d^(-1)(药物浓度3.0g/m L);贝那普利组灌服贝那普利0.90mg·kg^(-1)·d^(-1)。模型组(B)及WKY组每日灌服同体积的蒸馏水。共8周。检测干预前后大鼠血压,免疫组织化学法检测Bcl-2、Bax的表达。结果:干预前与空白组(WKY大鼠)比较,其他各组(SHR大鼠)收缩压、舒张压和平均血压明显高于同周龄的WKY大鼠(P<0.05);各组大鼠干预8周后再次进行血压测定,与模型组比较,各治疗组收缩压、舒张压和平均血压显著降低(P<0.05);镇肝熄风汤减轻了SHR的Bax表达(P<0.01),增加了Bcl-2的表达,其中中药高剂量组达到WKY水平(P>0.05)。结论:镇肝熄风汤不仅有明显的降压作用,而且在高血压病程中可抑制视网膜毛细血管的细胞凋亡。
Objective： To explore mechanism protective function of the Zhen＇gan Xifeng decoction on retina cells in spontaneously hypertensive rat. Methods： 48 SHR were randomly divided into four groups： the model group（B）, the benazepril group（C）, the low-dose Zhen＇gan Xifeng decoction observation group（D）, the Zhen＇gan Xifeng decoction high dose group（E）, the WKY group, each group 12 rats. The Zhen＇gan Xifeng decoction low-dose group was fed the zhen＇gan xifeng decoction 15 g·kg^-1·d^-1（concentration 1.5g/m L）; the zhen＇gan xifeng decoction high dose group was fed the zhen＇gan xifeng decoction 30g·kg^-1·d^-1（concentration 3.0g/m L）; the benazepril group was fed with 0.90 m g·kg^-1·d^-1. Model group（B） and WKY group were fed with daily volume of distilled water for 8 weeks. The blood pressure measured before and after the intervention, immunohistochemistry to detect Bcl-2, Bax expression. Results： Before the intervention compared with other groups（SHR rats） systolic, diastolic and mean blood pressure in the control group were significantly higher than the same week-old WKY rats（P〈0.05）; In 8 weeks after re-intervention blood pressure measurement, compared with the model group, the treatment group can significantly reduce systolic blood pressure, diastolic and mean blood pressure（P〈0.05）; the zhen＇gan xifeng decoction mitigate bax expression in SHR（P〈0.01）, increased Bcl-2 expression, which reached the high dose group WKY levels（P〈0.05）. Conclusion： The Zhen＇gan Xifeng decoction showed not only a significant antihypertensive effect, but also inhibited cell apoptosis of retinal capillaries in the course of hypertension.
出处
《中医临床研究》
2016年第5期1-3,共3页
Clinical Journal Of Chinese Medicine
基金
国家自然科学基金(81160477)
甘肃中医学院研究生创新基金(CX2014-12)
