摘要
目的:研究染料木素磺酸钠对去卵巢模型大鼠骨代谢的影响。方法:用去卵巢方法建立大鼠骨代谢紊乱动物模型,选择48只雌鼠,随机分为6组:正常对照组、骨质疏松模型组、阳性药物组、低剂量GSS组(0.1 mg·kg-1)、中剂量GSS组(0.3 mg·kg-1)、高剂量GSS组(1.0 mg·kg-1)。建模1周后,分别给予生理盐水、雌激素和不同剂量的染料木素磺酸钠,给药6周后,检测大鼠骨长度和骨重量指数,同时检测血清电解质、碱性磷酸酶(AKP)、骨钙素(BGP)和肝组织抗酒石酸酸性磷酸酶(Tr ACP-5b)等生化指标。结果:与模型组相比(1)GSS可以增高腰椎、股骨及胫腓骨重量指数,同时增大股骨和胫骨长度指数(P<0.001);(2)GSS可降低血清中骨钙素的含量,提高降钙素的含量(P<0.001);(3)GSS能升高血清AKP,降低Tr ACP-5b的含量(P<0.01)。结论:GSS可通过调节骨形成和骨吸收途径,从而改善去卵巢大鼠骨代谢的紊乱,对绝经后骨质疏松症可能起治疗作用。
Objective： To observe the effect of Genistein Sulfonate Sodium（ GSS） on bone metabolism in ovariectomized rat. Methods： 48 female SD rats were randomly divided into 6 groups： control group,ovariectomized model group,positive drug group,and difference dose of GSS groups（ 0. 1,0. 3,1. 0 mg·kg- 1）. After one week following ovariectomy,the rats were administrated saline,estradiol valerate or GSS by ig once a day. Six weeks later,the rats were sacrificed,their serum was taken to detect electrolytes,bone gla protein（ BGP）,calcitonin（ CT） and alkaline aphosphatase（ AKP）,and their liver tissues were make to observe tartrate-resistant acid phosphatase（ Tr ACP-5b）. Then the rats were detected bone length and mass index. Results： Compared with the model group,（ 1） GSS can increase lumbar spine,femur,tibia and fibula weight index,and increase the length index of the femur and tibia（ P〈0. 001）;（ 2） GSS can reduce serum BGP,and improve the content of calcitonin（ P〈0. 001）;（ 3） GSS can elevate serum AKP level and reduce liver Tr ACP-5b content（ P〈0. 01）. Conclusion： GSS can improve bone metabolism in ovariectomized rats by regulating bone formation and resorption way,and provide a potential therapeutic approach for postmenopausal osteoporosis.
出处
《赣南医学院学报》
2016年第1期13-16,共4页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
基金
国家自然科学基金(NO.31360250)
江西省卫计委(NO.20155465)
赣州市科技局(NO.GZ2014ZSF025)
关键词
染料木素磺酸钠
去卵巢大鼠
骨代谢
Genistein Sulfonate Sodium
ovariectomized rats
bone metabolism
