摘要
目的:观察黄荆子对哺乳期急性乳腺炎大鼠血小板水平及体积的影响。方法:24只SD孕鼠产后,随机分为6组分别为正常组、模型组、黄荆子高、中、低剂量组、阿莫西林(阳性对照组)每组8只,自然哺乳3 d后用于实验。除正常产后大鼠外,其他各组产后大鼠均予以复制哺乳期急性乳腺炎将其第3、4、5对乳房用75%乙醇擦拭后,经乳头管灌注2×1012CFΜ/m L金黄色葡萄球菌100μL/侧到大鼠第3、4、5对(双侧)乳腺内。正常对照组注射等容积的生理盐水。24 h后黄荆子高、中、低剂量组分别给予黄荆子混悬液12、6、3 g/kg灌胃,对照组予阿莫西林混悬液含药150 mg/kg灌胃,正常组及模型对照组均予等容量生理盐水灌胃,连续给予7 d。外周血采血,抗凝后上血象检测仪检测。结果:模型组大鼠外周血中血小板总数、平均体积及分布宽度均较正常组明显偏高(P<0.05),同时与模型组比较,黄荆子高、中、低剂量组均可明显降低乳腺炎大鼠血血小板总数、血小板平均体积及血小板分布宽度水平(P<0.05)。结论:黄荆子可降低哺乳期急性乳腺炎大鼠血小板数量、平均体积及分布宽度,提示黄荆子治疗哺乳期急性乳腺炎可能与降低血小板水平有关。
Objective： To observe the influence of Vitex negundo on platelet levels and volume of acute mammitis rats during the lactation period. Methods： 24 SD postpartum or pregnant rats were randomly divided into six groups： normal group,model group,Vitex negundo high,medium and low dose groups,amoxicillin（ positive control group）,eight in each group,natural feeding used in experiments in three days. In addition to the normal postpartum rats,other groups＇ postnatal rats were copying acute mammitis during the lactation period,using 75% ethanol to the breast after wiping,the nipple tube perfusion 2 × 1012CFΜ / m L staphylococcus aureus 100 μL / side to 3,4 and 5（ double side） mammary gland. Normal control group was administrated the physiological saline injections. 24 hours later,Vitex negundo high,medium and low dose groups were given Vitex negundo mixed suspension（ 12 g / kg,6 g / kg,3 g / kg） to fill the stomach and control group given amoxicillin suspension liquid containing medicine 150 mg / kg. The normal group and model control group were given physiological saline for 7 days. Result： Model group＇s peripheral blood platelet distribution,average volume and total width were definitely higher than those in the normal group（ P〈0. 05） and compared with model group,Vitex trifolia high,medium and low dose groups can significantly reduce mastitis rats＇ blood platelets,platelet total average volume and platelet distribution width（ P〈0. 05）. Conclusion： Vitex negundo fruit can reduce acute mammitis rats＇ lactation quantity,average volume and platelet distribution width. The treatment may be associated with lower levels of platelet.
出处
《中华中医药学刊》
CAS
北大核心
2016年第3期633-635,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81460679)
江西省科技支撑计划项目(2013BBG70018)
国家留学基金委地方合作项目(201408360106
201408360110)
江西省教育厅科技项目(GJJ13612)
江西中医学院校级课题(2012FC005)
关键词
黄荆子
哺乳期急性乳腺炎
血小板
数量
体积
Vitex negundo
acute mammitis during lactation
platelet
quantity
volume
