摘要
目的检测胃癌及癌旁组织中的赖氨酸特异性去甲基化酶1（LSDI）、E-钙黏蛋白的表达，探讨LSD1、E-钙黏蛋白与胃癌患者的临床病理特征、预后之间的关系，以及LSD1与E-钙黏蛋白表达的相关性。方法采用病例对照研究方法。收集2008年6月至2009年6月中南大学湘雅医院收治80例胃癌患者手术切除的胃癌组织及癌旁组织。采用免疫组织化学染色检测手术标本中LSD1、E-钙黏蛋白的表达情况。术后通过电话随访，了解患者生存情况。随访时间截至2015年6月。分析LSD1和E-钙黏蛋白的表达及其与胃癌患者临床病理特征的关系，LSD1和E-钙黏蛋白在胃癌组织中表达的相关性。分析LSD1、E-钙黏蛋白表达与胃癌患者预后的关系。计数资料的比较采用，检验，相关性采用Spearman等级相关分析，采用Kaplan—Meier法绘制生存曲线，Log-rank检验进行生存分析。结果LSDI在胃癌细胞及癌旁组织细胞中表达均定位于细胞核。其阳性表达率分别为67．5％（54／80）、43．8％（35／80），两者比较，差异有统计学意义（χ^2=9．141，P〈0．05）。E-钙黏蛋白在胃癌细胞及癌旁组织细胞中表达定位于细胞膜和细胞质。其阳性表达率分别为63．8％（51／80）、81．3％（65／80），两者比较，差异有统计学意义（χ^2=6．140，P〈0．05）。LSD1在不同的病理学分级的低、中、高分化的阳性表达率分别为83．3％（25／30）、76．0％（19／25）和40．0％（10／25），在TNM分期的Ⅰ、Ⅱ、Ⅲ、Ⅳ期中分别为37．5％（6／16）、72．7％（16／22）、71．9％（23／32）和90．0％（9／10），在无和有淋巴结转移中分别为36．4％（4／11）和72．5％（50／69），几者比较，差异均有统计学意义（χ^2=12．870，9．425，4．111，P〈0．05）。E-钙黏蛋白在低、中、高分化的阳性表达率分别为53．3％（16／30）、56．0％（14／25）和84．0�
Objective To investigate the expressions and relationship between lysine-specific demethylase 1 （ LSD1 ） and E-cadherin protein in gastric cancer tissues and adjacent normal tissues, and the correlation with the clinicopathological features and prognosis of patients with gastric cancer. Methods The ease-control study was adopted. The gastric cancer tissues and adjacent normal tissues were collected by surgical resection from 80 patients with gastric cancer who were admitted to the Xiangya Hospital of Central South University from June 2008 to June 2009. Expressions of LSD1 and E-cadherin protein were detected by immunohistochemistry （IHC）. The follow-up of telephone interview was performed to detect survival of patients till June 2015. Relationships between the expressions of LSD1 and E-eadherin protein and clinieopathologieal features or prognosis of patients were analyzed. Comparison of count data and correlation were analyzed by the chi-square test and Spearman rank correlation analysis. Survival curve was drawn using the Kaplan-Meier method, and survival analysis was done using the Log-rank test. Results Expressions of LSD1 in cancer tissues and adjacent normal tissues were located at the cell nucleus. The positive expression rate of LSD1 was 67.5% （54/80） and 43.8% （35/80） in cancer tissues and adjacent normal tissues, respectively, with a significant difference （χ^2= 9. 141, P 〈 0. 05）. Expressions of E-cadherin protein in cancer tissues and adjacent normal tissues were located at the cell membrane and cytoplasm. The positive expression rate of E-eadherin was 63.8% （51/80） and 81.3% （65/80） in cancer tissues and adjacent normal tissues, respectively, with a significant difference （χ^2 = 6. 140, P 〈 0.05 ）. The positive expression rate of LSD1 was 83.3% （25/30） , 76.0% （ 19/25 ） and 40.0% （ 10/25 ） in the low-, moderate- and high-differentiated tumors, 37. 5% （ 6/16）, 72.7% （ 16/22）, 71.9% （23/32） and 90.0% （9/10） in the Ⅰ, Ⅱ, Ⅲ and Ⅳ
出处
《中华消化外科杂志》
CAS
CSCD
北大核心
2016年第3期271-276,共6页
Chinese Journal of Digestive Surgery
基金
湖南省发改委科研项目（湘发改投资[2014]658）
湖南省自然科学基金项目（08JJ5009）
