摘要
利用反相微乳液法制备了壳聚糖-Ru(bpy)_3^(2+)-SiO_2复合纳米粒子,采用Nafion/MCNT复合膜技术实现了对复合纳米粒子有效而稳定的固定,从而制备了电化学发光传感器,实现了对尿酸的检测。在0.1 mol/L PBS缓冲溶液(p H 7.4)中,当尿酸与修饰电极作用15 min时,电化学发光强度与尿酸浓度(1.0×10^(-10)~1.0×10^(-5)mol/L)的负对数呈良好的线性关系,线性方程为IECL=-709.52-202.74lg C,相关系数R=0.9936,检出限为6.0×10^(-12)mol/L。传感器表现出良好的重现性与稳定性,对1.0×10^(-8)mol/L尿酸平行测定11次,发光强度的相对标准偏差为2.9%,测定尿酸实际样品的加标回收率在98.5%~103.5%之间。
Chitosan-Ru( bpy)3^(2+)-SiO2 composite nanoparticles( CRu S NPs) were prepared by reverse microemulsion method,and based on the Nafion / MCNT composite membrane technology,CRu S NPs were effectively and steadily immobilize on the surface of a glassy carbon electrode to prepare the electrochemiluminescence sensor for uric acid determination. In 0. 1 mol / L PBS( p H 7. 4) buffer solution,when the actuation duration between uric acid and the modified electrode was 15 min, the electrochemiluminescence showed a good linear relationship to the negative logarithm of uric acid concentration in the range of 1. 0 × 10^(-10)-1. 0 × 10^(-5)mol / L,the linear equation was IECL= -709. 52-202. 74 lg C and the correlation coefficient was 0. 9936 with a detection limit of 6. 0 × 10^(-12)mol / L. The ECL sensor exhibited excellent repeatability and stability,and the RSD for 11 times determination of 1. 0 × 10^(-8)mol / L uric acid was2. 9%. The recovery was 98. 5%-103. 5% in the determination of real Uric acid sample.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2016年第3期342-347,共6页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.30970696)
陕西省科技统筹创新工程计划项目(2012KTDZ02-02)
商洛市科技局计划项目(SK-2014-4)
陕西省尾矿资源综合利用重点实验室项目(14SKY-FWK06)资助~~