摘要
目的制备寡聚透明质酸衍生物oHA修饰的姜黄素-汉防己碱中药抗病毒脂质体,并对其进行体外释放和稳定性研究。方法用薄膜分散法制备了寡聚透明质酸衍生物修饰的姜黄素-汉防己碱脂质体,以粒径和包封率作为两个重要的参考指标,使用粒度仪测定了粒径,使用紫外分光光度法测定包封率。结果用Box-Behnken效应面优化法确定了最佳磷脂胆固醇比为2.5∶1,最佳姜黄素-汉防己碱比例为2.8∶1,寡聚透明质酸衍生物的用量为1.80%,通过最佳处方制备的脂质体的粒径是201.06 nm,包封率是70.94%。结论制备的脂质体具有良好的稳定性,均匀的粒径分布,证明了脂质体具有良好的体外释放活性,良好的稳定性,为进一步研究抗病毒联合机制奠定基础。
Objective To prepare hyaluronan oligosaccharides(oHA)modified antiviral liposome containing curcumin and tetrandrine,and study its release in vitro and stability.Methods The co -deliver antiviral liposomes was prepared by film dispersion method with particle size and entrapment rate as two important indicators,using particle size analyzer to de-termine the particle size and ultraviolet spectrophotometric method for the determination of entrapment efficiency.Results With Box - Behnken response surface optimization method,the optimal preparation (cholesterol and phospholipid ratio) was 2.5∶1.The best curcumin -tetrandrine proportion was 2.8∶1,and oHA was 1.80%.The particle size was 201.06 nm and entrapment efficiency was 70.94%.Conclusion The preparation method showed that liposomes had good stability,u-niform particle size distribution.It proved that the liposome had good in vitro release activity,good stability and had the po-tential use for antiviral nanocarriers.
出处
《药学研究》
CAS
2016年第3期157-160,共4页
Journal of Pharmaceutical Research
基金
国家自然科学基金项目(No.81302718、No.81573614)
烟台市科技发展计划项目(No.2015ZH079)
