摘要
目的通过联合检测红细胞输注前后调节性T细胞（CD4^＋CD25^＋）的变化和不规则抗体存在情况，探讨其是否有利于提高患者红细胞输注无效的检出率。方法选取渝北区人民医院红细胞输注无效患者31例，采用流式细胞术检测输血前后CD4^＋CD25^＋调节性T细胞变化，采用标准筛查细胞Ⅰ、Ⅱ、Ⅲ检测血清低效价、低亲和力不规则抗体。结果渝北区红细胞输注无效发生率为7．52％（31／412），内科系统患者红细胞输注无效构成比为77．42％（24／31），远大于外科系统患者的22．58％（7／31）（P=0．002），红细胞输注无效发生以肿瘤科、血液内科、感染科为主；红细胞输注无效患者CD4^＋CD25^＋调节性T细胞表达由（22．18±1．58）％下降至（16．57±1．77）％（P=0．023）。结论联合检测CD4^＋CD25^＋T细胞的变化和不规则抗体表达，有利于提高红细胞输注无效的检出率，预防和减少红细胞输注无效。
Objective To investigate whether the T cells （CD4^＋ CD25^＋） levels and the irregular antibodies screening cloud improve the diagnosis of invalid red blood cells transfusion. Methods Thirty-one patients with red blood cell invalid transfusion in the People＇s Hospital of Yubei District of Chongqing were selected. Flow cytometry was used to detect the changes of CD4^＋CD25^＋ regulatory T cells. Standard cells Ⅰ, Ⅱ, Ⅲ were used to screen on irregular antibodies in red blood cells （RBC） . Results The rate of invalid RBC transfusion was 7.52% （ 31/412 ） in Yubei District. The incidence rate of medical diseases was 77.42% （24/31）, much higher than surgical disease （22. 58% （7/31, P = 0. 002） , and the major departments were oncology department, hematological department and infectious department. The CD4^＋ CD25^＋ regulatory T cells were decrease from （22. 18±1.58） % to （16. 57±1.77） %（P=0. 023） . Conclusion Combin CD4^＋CD25^＋ regulatory T cells test and irregular antibody screening can help prevent and reduce the invalid transfusionof red blood cells.
出处
《中国综合临床》
2016年第2期113-115,共3页
Clinical Medicine of China
基金
重庆市渝北区科技项目（YBKW2013-2060403）
