摘要
目的:基于流式细胞术检测正常人的鼻黏膜组织中的淋巴细胞亚群(CD3+T、CD4+T、CD8+T、NK、CD19+B)的比例,初步探讨鼻局部黏膜免疫功能的意义,为鼻局部黏膜免疫性疾病的研究提供更多的参考。方法:用鼻黏膜刮匙获取21例正常人的鼻黏膜组织,并抽取其外周血2 m L。采用流式细胞术分别检测其鼻黏膜和外周血中的淋巴细胞亚群(包括CD3+T、CD4+T、CD8+T、NK、CD19+B)分别所占的比例。结果:鼻黏膜和外周血的淋巴细胞亚群存在很大差异,与外周血相比,CD3+T比例增加(t=15.34,P<0.0001),CD4+T比例降低(t=5.952,P<0.0001)、CD8+T比例增加(t=12.44,P<0.0001)、NK比例降低(t=4.865,P<0.0001)、CD19+B比例降低(t=15.56,P<0.0001),CD4+T/CD8+T降低。结论:流式细胞术可以用来检测鼻黏膜的淋巴细胞亚群,鼻黏膜的淋巴细胞亚群和外周血的淋巴细胞亚群存在很大差异,这种差异体现鼻黏膜组织独特的局部黏膜免疫功能,本方法为变应性鼻炎的研究提供了新的研究途径。
Objective： To detect the ratio of lymphocyte subsets （CD3^＋T, CD4^＋T, CD8^＋T, NK, CD19^＋B） in nasal mucosal tissues of normal people based on the method of flow cytometry, and investigate the meaning of topical nasal mucosal immune function to provide a new method for the study of topical nasal mucosal immune disease. Methods： 21 cases of nasal mucosal tissues from normal people were obtained with nasal mucosal wiper and the 2 mL peripheral blood was extracted; flow cytometry was performed to detect the respective ratios of lymphocyte subsets （CD3^＋T, CD4^＋T, CD8^＋T, NK, CD19^＋B） in these nasal mucosal tissues and peripheral blood. Results： There was significant difference in the ratio of lymphocyte subsets between nasal mucosal tissues and peripheral blood. Compared with the ratio of lymphocyte subsets in peripheral blood, CD3^＋T in the nasal mucosal tissues increased （P〈0.0001）, CD4^＋T decreased （P〈0. 0001）, CD8^＋T increased （P〈0.0001）, NK decreased （P〈0.0001）, CD19^＋B decreased （P〈 0.0001） and CD4^＋T/CD8^＋T reduced. Conclusions： Flow eytometry could be used to detect lymphocyte subsets in nasal mucosal tissues; significant differences exsisted in the ratio of lymphocyte subsets between nasal mucosal tissues and peripheral blood, which reflected the unique topical mucosal immune function of nasal mueosal tissues; this method provided a new way for the research of allergic rhinitis.
出处
《现代生物医学进展》
CAS
2015年第35期6969-6973,共5页
Progress in Modern Biomedicine
基金
上海市普陀区卫生系统自主创新资助项目(PTKW13-B03)
关键词
鼻黏膜
流式细胞术
淋巴细胞亚群
外周血
Nasal mueosa
Flow cytometry
Lymphocyte subgroup
Peripheral blood
