摘要
目的探讨用聚乳酸-羟基乙酸共聚物(PLGA)包载鱼尾葵花粉过敏原profilin蛋白的纳米疫苗特异性免疫治疗小鼠过敏性鼻炎的机制及疗效。方法将30只BALB/c小鼠随机分为5组:正常组、模型组、纳米疫苗治疗组、空白纳米治疗组及蛋白治疗组,每组6只。分别在第0、7、14天致敏小鼠,除正常组外,其余4组小鼠每次经腹腔注射50μg鱼尾葵花粉提取液和2mg Al(OH)3,正常组用PBS代替。致敏结束后,纳米疫苗治疗组、空白纳米组及蛋白治疗组分别使用PLGA-CmP纳米疫苗(含50μg profilin蛋白)、空PLGA纳米和50μg重组蛋白profilin经小鼠腹部皮下注射进行免疫治疗,每隔3d注射1次,共治疗5次。末次治疗后,取花粉粗浸液200μg分别滴鼻激发小鼠,每天1次,连续3d,激发结束后处死小鼠。小鼠激发后的30min内,观察并记录各组小鼠鼻部症状(鼻分泌物量、喷嚏次数及搔鼻)评分情况;检测血清中过敏原特异性抗体(IgE和IgG2a)及细胞因子(IL-4、IL-10和INF-γ)水平;观察鼻黏膜组织形态学变化。结果与模型组比较,蛋白治疗组及纳米疫苗治疗组小鼠鼻部症状评分、血清特异性IgE及IL-4水平均明显降低,纳米疫苗治疗组降低更明显,IgG2a、IL-10和INF-γ显著增加,纳米疫苗治疗组增加更明显(P<0.05或P<0.01)。结论以PLGA为佐剂的鱼尾葵花粉过敏原纳米疫苗可有效地抑制小鼠过敏性鼻炎的炎症反应,大大提高过敏原疫苗的治疗效果。
Objective To investigate the mechanism and efficacy of Caryota mitis profilin-loaded poly lactic-co-glycolic acid（PLGA）for the treatment of allergic rhinitis in mice.Methods Thirty BALB/c mice were randomly divided into 5groups,with 6mice in each group.Mice were sensitized by intraperitoneal injection of 50μg Caryota mitis pollen extract and 2mg Al（OH）3on days 0,7and 14 in model group,nanovaccine treatment group,blank nanotherapy group and profilin treatment group.Mice in normal group received PBS only.After sensitization,mice in nanovaccine treatment group,blank nanotherapy group and profilin treatment group were treated with subcutaneous injection of Caryota mitis profilin-loaded PLGA nanoparticles（containing 50μg profiling）,blank PLGA nanoparticles and 50μg recombinant protein profiling 5times once every 3days.After the last treatment,the mice were provocated with pollen crude infusion 200μg intranasally,respectively,once a day for three days,and the mice were sacrificed after provocation.Scores of nasal symptoms（amount of nasal secretion,number of sneezing,and scratching nose）were recorded within 30 minutes after provocation.In addition,serum levels of allergen-specific antibodies（IgE and IgG2a）and cytokines（IL-4,IL-10 and INF-γ）were measured and morphological changes in nasal mucosa were observed.Results Compared with model group,nasal symptom scores decreased,serum levels of IgE and IL-4reduced,and serum levels of IgG2 a,IL-10 and INF-γincreased in both profilin treatment group and nanovaccine treatment group,especially in nanovaccine treatment group（P〈0.05 or P〈0.01）.Conclusion Caryota mitis profilin-loaded PLGA nanoparticles can inhibit the inflammatory response and improve the efficacy of allergen vaccine in mice with allergic rhinitis.
出处
《南昌大学学报（医学版）》
CAS
2015年第6期1-5,共5页
Journal of Nanchang University：Medical Sciences
基金
国家自然科学基金(81302553)
广东省工程技术研究开发中心项目(2013158925)
广东省科技计划项目(2014A020212466)
南山区创新机构提升资助项目(KC2014ZDTS0004B
KC2014ZDTS0004B)
