摘要
目的比较3种检测方法——免疫胶体金包被法、ELISA检测法及PCR核酸检测法,测定登革热抗原的敏感性、特异性及其3种方法的优缺点。方法用免疫胶体金包被法和ELISA检测法及PCR核酸检测法,对来源于医院临床诊断的疑似登革热病例的血清进行抗原检测。结果 100份临床诊断疑似登革热病例的血清经过免疫胶体金包被法检测为阳性者再次用ELISA检测法及PCR核酸检测法测定抗原,检测后的阳性结果准确率均为100%,而100份免疫胶体金包被法检测为阴性的血清,用ELISA检测法及PCR核酸检测法测定抗原,结果出现了假阴性。结论通过比较免疫胶体金包被法、ELISA检测法及PCR核酸检测法检测登革热抗原,使用ELISA检测法及PCR核酸检测法测定登革热抗原的敏感性较强,准确度很高并具有可排除干扰因素的优点。
Objective To compare the advantages and disadvantages of three methods,including immune colloidal gold package method,ELISA assay and PCR assay,on sensitivity and specificity in the determination of dengue fever antigen. Methods Immune colloidal gold package,ELISA assay and PCR assay method were used to determine the serum antigen of the suspected dengue cases from hospital clinical diagnosis. Results 100 cases of positive serum from suspected dengue fever cases diagnosed in clinic detected with immune colloidal gold package method were determined by ELISA assay and PCR assay,after testing,the positive result of accuracy rate was 100%,and 100 cases of negative serum from suspected dengue fever cases diagnosed in clinic detected with immune colloidal gold package method were determined by ELISA assay and PCR assay,and false negativeness in results were detected. Conclusion By comparing the determination of dengue fever antigen with immune colloidal gold package method,ELISA assay and PCR assay,the sensitivity of ELISA assay and PCR test is stronger in the dengue fever antigen detection. The accuracy is very high and it has the advantage of eliminating interference factors.
出处
《中国卫生检验杂志》
CAS
2016年第3期358-359,共2页
Chinese Journal of Health Laboratory Technology
