摘要
于2013年11月至2014年4月,采用微生物磷酸脂肪酸(PLFA)分析方法,以川西高山草甸代表物种黄花亚菊(Ajania nubigena)和黑褐苔草(Carex atrofusca)凋落物为研究对象,在雪被形成期、雪被稳定期和雪被融化期研究了凋落物分解过程中土壤动物对微生物群落结构及其多样性的影响.结果表明:(1)整个冬季,土壤动物增加了黄花亚菊凋落物分解过程中微生物总P LFA含量、真菌PLFA含量以及细菌PLFA含量,提高了其真菌/细菌比例,减少了黑褐苔草中微生物总PLFA含量、真菌PLFA含量以及细菌PLFA含量,降低了其真菌/细菌比例;(2)在雪被形成期,黄花亚菊凋落物中土壤动物显著增加了真菌表征脂肪酸18:2ω6,9c的含量,但降低了细菌表征脂肪酸16:0、16:1ω9c、18:1ω7c的含量.黑褐苔草凋落物中土壤动物显著增加了细菌表征脂肪酸18:1ω7c的含量,但降低了真菌表征脂肪酸18:2ω6,9c的含量.雪被融化期,土壤动物显著增加了黑褐苔草凋落物中细菌表征脂肪酸16:1ω9c的含量,降低了细菌表征脂肪酸a15:0和微真核生物表征脂肪酸18:3和20:4的含量;(3)土壤动物对凋落物中微生物多样性的影响主要在雪被融化期,增加了黄花亚菊凋落物中微生物多样性、均匀性、丰富度和优势度指数,降低了黑褐苔草凋落物中微生物多样性和均匀性指数,增加了黑褐苔草凋落物中微生物丰富度和优势度指数.这些结果表明,冬季恶劣环境条件下高山草甸土壤动物仍然对微生物的数量、群落结构和多样性具有显著的影响.
Interactions between soil fauna and microbial community in litter might ultimately manipulate the process of litter decomposition in terrestrial ecosystems.Little is so far known about such interactions during winter litter decomposition in cold biomes.This study aimed to understand the effects of soil faunal community on winter microbial community structure in folia litter during winter decomposition in cold biomes.Litterbags with foliar litter of Ajania nubigena and Carex atrofusca were respectively placed on the soil surface in an alpine meadow of Zhegu Mountain,Miyaluo Nature Reserve located in the upper reaches of Yangtze River and the eastern Tibet-Qingzang Plateau.After being incubated in situ on Nov.13^th,2013,the litterbags were taken back on Dec.6^th,2013（Snow forming period,SFP）,Mar.5^th,2014（Snow stable period,SSP） and Apr.5^th,2014（Snow melting period,SMP）,respectively.The soil faunal and microbial communities in litter were measured by funnel method and phospholipid fatty acid（PLFA）,respectively.The results showed that soil fauna generally increased the contents of total PLFA,fungal PLFA,and bacterial PLFA of microbial community in A.nubigena foliar litter,and raised the ratio of fungal PLFA to bacterial PLFA,but those in C.atrofusca foliar litter showed the opposite trends,implying that the interactions between soil faunal community and microbial community in winter litter decomposition depend on plant species.In the SFP,soil fauna increased fungal 18：2ω6,9c PLFA content in A.nubigena foliar litter,but decreased bacterial 16：0,16：lω9c and18：1ω7c PLFA contents;soil fauna in C.atrofusca foliar litter increased bacterial 18：1ω7c PLFA content,but decreased fungal18：2ω6,9c PLFA content.In the SMP,soil fauna in C.atrofusca foliar litter increased bacterial 16：1ω9c PLFA content,but decreased the contents of bacterial a15：0 PLFA,and micro-eukaryotic 18：3 and 20：4 PLFA.More significant effects of soil faunal community on microbial community structure in both foliar
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2016年第1期27-34,共8页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(31170423和31270498)
国家“十二五”科技支撑计划(2011BAC09B05)
四川省杰出青年学术与技术带头人培育项目(2012JQ0008和2012JQ0059)
中国博士后科学基金(2012T50782)
四川省教育厅青年基金(2013SZB0553)资助~~
