摘要
目的探讨食管癌中鼠类肉瘤滤过性毒菌致癌同源体B1（BRAF）的基因突变和蛋白质表达情况。方法纳入2014年2月至2015年9月接受手术治疗的75例食管癌患者，取其癌组织、近癌组织和远癌组织，检测BRAF基因的突变和BRAF蛋白质的表达情况，分析BRAF蛋白质阳性表达与食管癌患者临床病理特征的关系。计数资料比较采用卡方检验。结果在食管癌组织中未检测到BRAF基因第11和15外显子存在突变。75份食管癌组织中，5份Ⅲb期标本在第11外显子上增加了1个碱基C或T。75份食管癌组织中，46份（61.3%）BRAF蛋白质表达阳性；57份近癌组织中，9份（15.8%）BRAF蛋白质表达阳性；75份远癌组织中，5份（6.7%）BRAF蛋白质表达阳性；3组间比较差异有统计学意义（χ^2＝61.098，P〈0.05）。TNM分期Ⅰ、Ⅱ、Ⅲ期食管癌患者的BRAF蛋白质阳性率分别为21.7%（5/23）、70.8%（17/24）和85.7%（24/28），有和无淋巴结转移患者的BRAF蛋白质阳性率分别为81.6%（31/38）和40.5%（15/37）；BRAF蛋白质表达阳性与TNM分期和肿瘤淋巴结转移有关（χ^2＝23.136、13.313，P均〈0.01），与患者性别、年龄和肿瘤分化程度无关（P均〉0.05）。结论食管癌组织中BRAF基因第11外显子上有碱基插入情况，但未见突变发生。BRAF蛋白质在食管癌中呈高表达，与TNM分期和淋巴结转移情况有关，可作为判断食管癌恶性程度和患者预后的指标。
Objective To estimate the gene mutation and the protein expression of v-Raf murine sarcoma viral oncogene homolog B1 （BRAF） in esophageal cancer. Methods From February 2014 to September 2015, 75 patients with esophageal cancer who received operation were enrolled. Tissues of cancer, adjacent to cancer and far from cancer were taken. The mutation and protein expression of BRAF were detected. The relationship between BRAF protein positive expression and clinical characteristics of patients with esophageal cancer was analyzed. The enumeration data was compared by chi-square test. Results The mutation of exon 11 and exon 15 of gene BRAF was not found in the tissues of esophageal cancer. Among 75 esophageal cancer, a base C or T inserted in the exon 11 was found in five Ⅲb TNM stage cases, and the expression of BRAF at protein level was positive in 46 cases （61.30/00）. Among 57 tissues adjacent to cancer, nine cases （15.8 % ） was BRAF positive at protein level. Among 75 tissues far from cancer, five（6.7%） was BRAF positive at protein level. The difference among three groups was statistically significant （χ^2= 61. 098,P〈0.05）. The positive rates of BRAF expression at protein level in patients with esophageal cancer at Ⅰ , Ⅱ and Ⅲ TNM stage were 21. 7% （5/23）, 70. 8% （17/24） and 85.7 %（24/28）, respectively. The positive rates of BRAF expression at protein level in patients with and without lymph node metastasis were 81. 6% （31/38） and 40. 5%（15/37）. The positive expression of BRAF at protein level was related with TNM stage and lymph node metastasis （χ^2=23. 136 and 13. 313,both P〈0.01）, however it was not related with gender, age and the degree of tumor differentiation （all P〉0.05）. Conclusions There is base insertion in the exon 11 of gene BRAF in esophageal cancer, but gene mutation is not found. BRAF is highly expressed in esophageal cancer, which is related with TNM stage and lymph node metastasis, and BRAF could be an indicator of assessment of degree
出处
《中华消化杂志》
CAS
CSCD
北大核心
2016年第2期73-77,共5页
Chinese Journal of Digestion
基金
河北省卫生厅医学科学研究计划课题（20130236、20150353）
