摘要
为适应油菜机械化生产,提高油菜生产效益,挖掘甘蓝型油菜的抗裂角基因资源,基于候选基因途径,根据拟南芥FUL基因的序列设计引物,在甘蓝型油菜抗裂角品系R1-1中扩增Bn FUL基因。序列分析结果表明,Bn FUL基因的ORF长度为726 bp,编码241个氨基酸,具有典型的MADS转录因子结构域。氨基酸序列的比对结果显示十字花科植物FUL蛋白的功能域MADS-box和K-box的氨基酸序列变异较小,多数变异集中在3'末端的非domain区。在拟南芥中过表达Bn FUL基因后发现Bn FUL基因具有一因多效作用,其不仅能增强拟南芥角果的裂角抗性,还能促进拟南芥提前开花,并产生复果现象,在一些转基因拟南芥植株中三个角果同时着生在一个果柄上。这些结果表明Bn FUL可能与甘蓝型油菜花和角果的发育相关。
To adapt to machine harvesting,pod shatter resistance of oilseed rape is essential. To better understand the resistance genetics,a 726 bp open reading frame（ ORF） of Bn FUL coding 241 amino acids was cloned from a pod shatter resistant line R1- 1（ Brassica napus） based on genome sequence of At FUL in Arabidopsis thaliana. The deduced Bn FUL protein contained a typical MADS- box and K- box conserved domain. Multiple sequence alignments showed that FUL protein sequences from different species were highly conserved within Cruciferae family,especially in MADS- box and K- box domains. Sequence variation of FUL homologs mainly occurred at 3＇- end in Cruciferae. Ectopic expression of Bn FUL in Arabidopsis thaliana resulted in pleiotropic phenotypes,including elevated resistance to pod shattering,earlier flowering and single peduncle with 3 siliques. These demonstrated that Bn FUL gene might control flower and fruit development in oilseed rape.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2016年第1期13-18,共6页
Chinese Journal of Oil Crop Sciences
基金
国家油菜现代产业技术体系建设(CARS-13)
中国农业科学院科技创新工程
湖北省农业科技创新中心项目
