摘要
为探讨枯草芽孢杆菌（Bacillus subtilis）在鱼类养殖池塘中的生态作用,采用直接往养殖水体中投放该制剂的方法,研究分析微生物数量及其与环境因子的相关关系。结果显示,枯草芽孢杆菌,实验池数量为0.35×10^3^1.45×10^3cfu/m L,对照池为0.04×10^3^0.08×10^3cfu/m L;浮游植物生物量,实验池为0.094^1.521 mg/L,对照池为0.103^0.763 mg/L,实验池中枯草芽孢杆菌数量和浮游植物生物量均高于对照组。试验鱼塘中枯草芽孢杆菌与硅藻数量呈显著正相关,相关系数0.844（P〈0.05）;当溶氧≥6 mg/L时,枯草芽孢杆菌与亚硝酸盐氮含量呈显著负相关,相关系数-0.915（P〈0.05）。溶氧过低（〈2 mg/L）时,枯草芽孢杆菌对亚硝酸盐氮、氨氮没有明显的降解作用;溶氧≥6 mg/L时,对亚硝酸盐氮、氨氮的降解作用明显。研究表明,投放适量浓度的枯草芽孢杆菌能有效改善养殖水体状况,对水质起到进一步净化作用。
To discuss the ecological effects of micro-ecological agents of Bacillus subtilis into marine fish pond,the relationship between microorganism quantity and environmental factors was investigated in marine fish pond by artificially adding method. The results showed that the quantity of Bacillus subtilis amounted to 0. 35 × 103-1. 45 × 103 cfu / m L in the experiment group,and 0. 04 × 103-0. 08 × 103 cfu / m L in the control group; the phytoplankton biomass（ 0. 102 6-0. 763 2 mg / L in the experiment group and 0. 093 6-1. 520 9 mg / L in the control group） were both significantly higher in the experiment group than those in the control group. In the experiment group; the Bacillus subtilis amounts were significantly positive correlative with the diatom abundance（ correlation coefficient = 0. 844,P〈0. 05）,but those were significantly negative correlative with the nitrite nitrogen concentration（ correlation coefficient =- 0. 915,P〈0. 05）,if the dissolved oxygen concentration was more than 6 mg / L. In addition,if the dissolved oxygen concentration was too low（ 2 mg / L）,there was no degradation effect of Bacillus subtilis on ammonia nitrogen and nitrite nitrogen in the seawater,but there was a significant degradation effect of Bacillus subtilis on ammonia nitrogen and nitrite nitrogen when the dissolved oxygen concentration reached to 6 mg / L or more. Thus it can be inferred that the appropriate concentration of Bacillus subtilis can effectively improve the condition of aquaculture water,and further purify the water quality.
出处
《渔业现代化》
北大核心
2016年第1期1-6,共6页
Fishery Modernization
基金
国家海洋公益性行业科研专项(201205025)
关键词
枯草芽孢杆菌
菌落数
溶氧
海水鱼池
氨氮:亚硝酸盐氮
Bacillus subtilis
colony number
dissolved oxygen
marine fish pond
ammonia nitrogen
nitrite nitrogen
