摘要
目的研究复方苗药通窍化栓方各剂量组对MCAO大鼠额叶、小脑部位血管内皮生长因子（VEGF）表达的影响.方法：健康SD大鼠150只,随机分为5个组（n=30）：模型组、阳性药物对照组、苗药高、中、低剂量组,每组30只.各组再分别根据缺血再灌注时间,随机分为缺血再灌注后6h、24h两个亚组,每组各15只,依次编为A、B、C、D、E、F、G、H、I组.苗药组： 分别从术前3天开始按高、中、低剂量的苗药灌胃,每日1次.阳性药物对照组、模型组按同样方法予以等量复方丹参注射液、生理盐水灌胃.采用免疫组化法检测不同组VEGF在MCAO 侧梗死额叶、小脑组织微血管内皮细胞的阳性表达数目.结果：不同组VEGF在MCAO侧梗死额叶、小脑组织微血管内皮细胞比较C、D组阳性表达数目最高,与其他组比较差异均有统计学意义（P〈0.05）.结论：MCAO大鼠梗死侧远隔部位在MCAO后6h苗药中高剂量组VEGF阳性表达数目最高,急性脑梗死后早期应用中高剂量通窍化栓方对改善神经功能缺损具有重要作用.
ObjectiveTo investigate the each does of Tongqiao Huashuan Formula for vascular endothelial growth factor expression on middle cerebral artery occlusion（MCAO） rats infarction area hematogenously. Methods：150 healthy adult SD rats were randomly divided into 5 experiment groups（n =30）：model group, positive drug control group, Miao medicine high, medium and low dose group（n=30）; According to ischemia reperfusion time again, Each group were randomly divided into 6 hour, 24 hour after ischemia reperfusion to two subgroups（n=15）, in turn make up for A, B, C, D, E, F, G, H, I 9 groups. Miao medicine group： Respectively from preoperative 3days according to the high, medium and low dose of Miao medicine to gavage, 1 times a day; Positive control group, model group： Using the same method the equal amount compound danshen injection, normal saline to gavage. Using immunohistochemical method to detect the number of positive expression of microvascular endothelial cells of the different set of VEGF in MCAO side infarction frontal lobe and cerebellum. Results：Different groups of VEGF in MCAO side infarction frontal lobe and cerebellum, tissue microvascular endothelial cells is the highest number of group C, group D positive expression, compared with the other groups were statistically significant difference （P 〈 0.05）. Conclusion：Lateral hematogenously MCAO rats infarction in 6 h after MCAO miao medicine middle and high dose group of the highest doses of VEGF positive expression, middle and high doses of the bolt plays an important role in improving neurologic deficits, when early after acute cerebral infarction.
出处
《世界中医药》
CAS
2015年第A02期1237-1239,共3页
World Chinese Medicine
基金
贵州省优秀科技教育人才省长专项资金项目（自然科学类）黔审专校字（2010）144号
关键词
中医药
通窍化栓方
急性脑梗死
血管内皮生长因子
实验研究.
Traditional Chinese Medicine
Tongqiao Huashuan Formula
Acute Cerebral Infarction
vascular endothelial growth factor
experimental study.
