摘要
建立了一种同时测定烟草中吡虫啉和多菌灵农药残留的高效液相色谱方法。样品经丙酮超声提取,Cleanert CN固相萃取柱净化、富集。采用甲醇-0.02mol·L^(-1)磷酸盐缓冲溶液(pH=7.4)为流动相,配备ODS-C18柱、紫外检测器的高效液相色谱(HPLC)仪对待测组分进行了分离和测定。结果表明:吡虫啉和多菌灵在0.2~20μg·mL^(-1)的范围内线性良好,相关系数分别为0.999 9和0.997 3。多菌灵和吡虫啉的添加回收率分别在87.3%~95.7%和84.7%~94.5%之间,相对标准偏差(RSD)均小于3.5%。
A analytical method of carbendazim and imidacloprid pesticide residues in tobacco by high performance liquid chromatography （HPLC） was developed. Samples was extracted with acetone and cleaned up through the Cleanert CN SPE. The HPLC conditions were ODS-C18 colum, methanol-0.02 mol·L^-1 phosphate buffered saline （pH= 7.4） as mobile phase, and UV detection at 278 nm. The results showed that carbendazim and imidaeloprid in the concentration range of 0.2-20μg·mL^-1 have a good linear relationship, the correlation coefficient are 0. 997 3 and 0. 999 9 respectively. The fortified recoveries of carbendazim and imidaclorpid in tobacco samples were 87.30%-95.7% and 84.7%-94.5%, respectively. Relative standard deviation was both less than 3.5%.
出处
《青岛科技大学学报（自然科学版）》
CAS
2016年第1期26-31,共6页
Journal of Qingdao University of Science and Technology:Natural Science Edition
关键词
高效液相色谱法
吡虫啉
多菌灵
烟草
high performance liquid chromatography
carbendazim
imidacloprid
tobacco
