摘要
目的:探讨不同临床表现形式HBV感染者T细胞免疫应答功能。方法:分离外周血单核细胞,流式细胞术检测患者T细胞亚群,酶联免疫斑点技术检测患者T细胞对HBV抗原的免疫应答反应。结果:与急性乙型肝炎患者(A组)比较,慢性肝炎感染患者(B组)和无症状携带者(C组)CD4+T细胞亚群频率均显著降低(P<0.01),而3组CD8+T细胞亚群频率差异无统计学意义(P>0.05);与A组比较,B组和C组CD4+/CD8+比值均明显下降(P<0.01);A组斑点形成细胞显著少于B组和C组(P<0.01);B组斑点形成细胞亦显著少于C组(P<0.01)。结论:不同临床表现形式的患者T细胞免疫存在不同,而T细胞亚群之间的平衡失调可能导致机体免疫紊乱,不能清除病毒,是HBV感染患者表现不同临床症状的原因。
Objective： To explore the T cell immune response function in different clinical manifestation patients. Methods： The peripheral blood mononuclear cells were isolated. The T cell subgroup was detected using flow cytometry, and the immune response of T cell to HBV antigen protein was detected using enzyme-linked immunespots assay. Results ： Compared with the acute hepatitis patients （group A）, the frequency of CD4^T cell subgroup in chronic hepatitis patients （group B） and asymptomatic carriers （group C） were significantly lower（ P 〈 0.01 ）, and the differences of the frequency of CD8^T cell subgroup in 3 groups were not statistically significant （ P 〉 0.05 ）. Compared with group A, the ratios of CD4^/CD8^ in group B and group C were decreased （ P 〈 0.01 ）. The spof forming call in group A was significantly less than that in group B and group C （ P 〈 0. 01 ）, and the spof forming call in group B was significantly less than that in group C （ P 〈 0.01 ）. Conclusions ： T cell immune in patients with different clinical manifestations is different, and the dysequilibrium of T cell subgroup may lead to immune disorders, and can not clear the virus, which is the cause of different clinical symptoms.
出处
《蚌埠医学院学报》
CAS
2015年第12期1621-1623,共3页
Journal of Bengbu Medical College
基金
延安大学高水平大学学科建设基础医学资助项目(2013SXTS02)
