摘要
对美味牛肝菌菌丝体液体深层发酵富硒条件进行优化,考察p H值、温度、转速、培养基中硒浓度等因素对富硒率的影响。结果表明:美味牛肝菌菌丝体富硒的适宜培养条件为:温度25℃,初始p H 5.0,转速150 r/min,培养基中添加亚硒酸钠的质量浓度为50 mg/L。在此条件下,富硒率最大达26.32%。分离获得富硒美味牛肝菌菌丝体中的粗多糖,对其清除羟自由基,抑制超氧阴离子,清除DPPH·自由基的能力进行了研究。对羟自由基清除作用的EC50值:VC 0.42 mg/m L,富硒菌丝体多糖0.54 mg/m L;对超氧阴离子清除作用的EC50值:VC0.31 mg/m L、富硒菌丝体多糖0.38 mg/m L、菌丝体多糖0.59 mg/m L;对DPPH·自由基的清除作用的EC50值:VC0.35 mg/m L,富硒菌丝多糖0.59 mg/m L。美味牛肝菌菌丝体多糖具有一定的抗氧化活性,并在设定的浓度范围呈剂量效应。富硒多糖抗氧化活性高于没有富硒培养的菌丝体多糖。
In this paper, the optimizing cultivation urn, using submerged liquid culture, were studied. The conditions for a Selenium-accumulating of Boletus edulis myceliinfluence factors in cultivation medium on Selenium accumulation rate including pH value, temperature, rotational speed, concentration of Selenium were optimized. The results showed that the optimum conditions were： temperature 25 ℃, initial pH 5.0, rotational speed 150 r·min^-1, and the concentration of Selenium in cultivation medium 50 mg·L^-1. Under such conditions, the selenium-rich rate can reach up to 26.32%. The crude polysaccharide was isolated from Boletus edulis mycelium cultured by Se in medium, and its antioxidant abilities in scavenging hydroxyl radical, inhibiting superoxide anion, scavenging DPPH radical were studied. The EC50 values of VC, and Selenium-accumulating mycelium polysaccharide were obtained as follow, 0.42 mg·mL^-1 and 0.54 mg·mL^-1 with scavenging hydroxyl radical, 0.31 mg·mL^-1, 0.38 mg·mL^-1 with scavenging superoxide anion, 0.35 mg·mL^-1, 0.59 mg·mL^-1 with removing DPPH. radical, respectively. The EC50 values of mycelium polysaccharide to scavenging hydroxyl radical was 0.59 mg ·mL^-1. Conclusion： Boletus edulis mycelium polysaccharides exerted antioxidant activities in a concentration-dependent manner, and the antioxidant activities of selenium-rich mycelium polysaccharides were higher than those mycelium polysaccharides without selenium-rich cultured.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2015年第12期91-98,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
财政专项-中央高校基本科研业务费资助项目(DC201501020301)
2012年度国家民委科研立项(12DLZ004)
辽宁省自然科学基金项目(2015020676)
关键词
美味牛肝菌
富硒率
菌丝体多糖
抗氧化性
Boletus edulis
selenium accumulation rate
mycelium polysaccharide
antioxidant activity
