摘要
目的探讨红色毛癣菌几丁质合酶(chitin synthase,CHS)m RNA在甲真菌病体外模型中的改变及其在致病中的作用。方法构建甲真菌病体外模型,随机分为实验组和对照组(未加甲板),分别于培养的第0,1,3,5,7,14和20天提取红色毛癣菌总RNA,采用实时荧光定量PCR检测不同时间点CHS m RNA的表达水平,并进行统计学分析。结果对照组CHS m RNA表达水平极低,实验组在红色毛癣菌感染甲板1d,3d,5d表达升高,7d表达达高峰,此后渐渐下降,但仍高于对照组。各时间点与对照组相比,除CHS 3d外,其余差异均有统计学意义(P<0.05)。结论 CHS m RNA在甲真菌病体外模型中有较高表达,在甲真菌病的致病中可能起重要作用。
Objective To investigate the expression and pathological role of CHS m RNA in vitro onychomycosis model.Methods Total RNA of Trichophyton rubrum in the treated group and control group（ non-infected nails）was extracted from cultured onychomycosis model at different time points（0d,1d,3d,5d,7d,14 d and 20d）.The expression of CHS m RNA at different time points were detected by real-time fluorescence quantitative PCR method,respectively,and performed statistical analysis. Results The expression of CHS m RNA was very low in control group. In treated group,the expression of CHS m RNA increased at 1d,3d and 5d,and reached a peak at 7d. Then the expression decreased gradually,but still higher than control group. Compared to the control group,the expression levels of CHS m RNA at each time points were significantly higher than the treated group with the exception of 3d（ P 〈0. 05）. Conclusion In vitro onychomycosis model,the expression levels of CHS m RNA are higher in cultured keratin from nail. CHS may play an important role in the pathogenesis of onychomycosis.
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2016年第3期233-235,256,共4页
The Chinese Journal of Dermatovenereology
基金
解放军南京军区医学科技创新课题(09MA065)
关键词
红色毛癣菌
几丁质合酶
实时荧光定量PCR
Trichophyton rubrum
Chitin synthase
Real-time fluorescence quantitative PCR
