摘要
[目的]探讨ABCC2在胆管细胞癌中的表达水平及其临床意义。[方法]收集湖北中医药大学附属医院病理科2010~2015年20例胆管细胞癌组织及其配对的10例远癌组织,利用实时荧光定量PCR和Western blot方法检测两组ABCC2的表达,比较分析ABCC2表达水平与胆管细胞癌的临床分期以及淋巴结转移的关系。[结果]ABCC2基因或蛋白在胆管细胞癌组织和远癌组织中均有不同程度的表达,且在癌组织的表达量显著低于远癌组织（P〈0.05）;此外,ABCC2表达在胆管细胞癌的不同淋巴结转移之间没有显著差异。[结论]ABCC2在胆管细胞癌中低表达（mRNA表达水平P=0.003,蛋白表达水平P=0.0031）,且与淋巴结转移之间没有显著差异,可能在胆管细胞癌的发生发展中起一定作用,有助于胆管细胞癌的临床诊断和病情评估。
[ Objective] To explore ABCC2 expression level in Intrahepatic cholangiocarcinoma （ICC） and its clinic signifi- cance. [ Methods] Select the 20 cases of eholangiocareinoma tissues from 2010 to 2015 in the Affiliated Hospital of Hubei tra- ditional Chinese Medicine University, and 10 cases of corresponding non -cancerous tissues were also collected for comparison. ABCC2 gene and protein expression were detected in two groups using Quantitive real - time PCR （qPCR） or Western blot method, and then compared and analyzed the relationship between ABCC2 expression and clinical staging of ICC or lymph node metastasis. [ Results ] ABCC2 gene and protein were expressed with different degrees in ICC and non -cancerous tissues, and its expression in ICC tissues was significant lower than that in non -cancerous tissues （P 〈 0.05 ）. However, there was no signifi- cant difference in ABCC2 expression in ICC and lymph node metastasis. [ Conclusion ] The low expression of ABCC2 gene or protein in ICC tissues （mRNA levels, P = 0. 003; Protein levels, P = 0. 0031 ） and no significance was found in lymph node metastasis,which may play important roles in the occurrence and development of cholangioearcinoma, and to further help clini- cal diagnosis and disease assessment of Intrahepatie cholangiocareinoma.
出处
《生物技术》
CAS
CSCD
北大核心
2015年第6期581-585,共5页
Biotechnology
基金
国家自然科学基金项目("转ABCC2和ABCC4基因斑马鱼抗毒物积累特性研究"
No.31172395)
国家科技支撑计划项目("环境作用因素关键检测技术研发-环境污染物孕前风险预警关键技术研究"
No.2013BAI12B01-3)资助
关键词
胆管细胞癌
ABCC2表达
淋巴结转移
intrahepatic cholangiocareinoma, ABCC2 expression, lymph node metastasis
