摘要
目的探索抑郁症大鼠海马区磷酸化微管相关蛋白-2(pMAP-2)表达和与之对应组织形态学上的变化。方法将成年大鼠随机分为对照组、模型组,采用慢性不可预见性温和应激方法建立抑郁大鼠模型,采用糖水偏好实验、旷场实验以及Morris水迷宫对其进行行为学检测,尼氏染色法观察海马神经元形态学改变.Western blot检测1、7、14、28d对照组、模型组海马组织中pMAP-2蛋白表达水平。结果与对照组相比,糖水偏好实验模型组糖水消耗量和糖水偏好百分比分别降低(P<0.05),模型组在旷场实验、中央活动时间、行走总里程、以及直立次数和修饰行为等均降低(P<0.05),Morris;水迷宫模型组相对于对照组平均逃避潜伏期增高(P<0.05),相比于对照组,CUMS后1d模型组大鼠海马p-MAP2蛋白表达较少(P<0.05),但是从第7天开始持续的高表达(P<0.05),在14 d时这种高表达到了最高峰(P<0.05),并且这种增高在第28 d时依然非常明显(P<0.05)与对照组比较,模型组海马神经元体积萎缩,数量减少。结论 pMAP-2在抑郁模型大鼠海马出现先降低后增高的现象,可能与海马体积异常变化有关。
Objective To explore the microtubule-associated protein 2 （pMAP-2） expression in hippocampal of the depression rats and the corresponding changes of histomorphology. Methods The SD rats were randomly divided into contol group and model group, the depression rat model was produced by giving the chronic unpredictable mild stess （CUMS）. The depressional behabior was examined by useing surose preference test,tail-suspension test and Morris water maze. The morphological change of hippocampal neurons was carried out by Nissl staining. At 1, 7, 14, 28d after modeling,the expression of pMAP-2 in hippocampal of control group and model group were detected by the Western Blot. Results Compared to control group, the consumption of sucrose and percentage of sucrose preference of rats in model group were significantly lower, while in open field test, the total walking distance, central activity time, up-right times and grooming behavior in model group were also lower than that in control group （P 〈0.05 ）. In the Morris water maze, the model group average escape latency period is higher than that in control group （ P 〈 0.05 ）. Compared with the Sham group, the expression of p-MAP2 in model group was obviously lighter in 1 d after CUMS （P 〈 0. 05 ） ,but continuous high expression from 7 d （P 〈 0.05 ）, reached its highest point in 14 d （ P 〈 0.05 ）, and the high expression in 28 d is still very obvious （ P 〈 0.05 ）. Compared to control group, the neuroanl body was smaller, and the number of neurons was decreased. Conclusion The expression level of pMAP-2 protein in the depression model of hippocampus of rats decreases firstly and then increases, which is probably related to the change in the hippocampal volume abnormal of depression.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第3期355-357,362,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金项目(编号:81201048)
关键词
抑郁症
海马
微管相关蛋白
大鼠
depression
hippocampus
microtubule-associated protein
rats
