摘要
目的:探讨柴胡皂苷对抑郁模型大鼠行为及海马神经元细胞凋亡的保护作用。方法:采用随机数字表法将60只SD雄性大鼠分为对照组、模型组、氟西汀组和柴胡皂苷组,每组15只。采用慢性不可预见性应激刺激(CUMS)加孤养方式复制抑郁模型。于造模第2天开始,对氟西汀组和柴胡皂苷组大鼠进行灌胃给药。在实验的第1、7、14、21天,通过糖水消耗量实验检测大鼠行为学改变。采用RT-PCR法对各组大鼠脑海马神经元JNK、Bad m RNA进行定量分析。结果:对照组、柴胡皂苷组、氟西汀组第14及21天糖水偏爱度均高于模型组,JNK、Bad m RNA表达量均低于模型组,比较差异均有统计学意义(P<0.01)。结论:柴胡皂苷可以有效控制大鼠抑郁症状,其机制可能与降低海马组织中JNK、Bad蛋白表达有关。
Objective： To investigate the protective effect of Saikosaponin on the behavior and the apoptosis of hippocampus neurons in depression model rats.Method： 60 SD rats were randomly divided into the control group, the model group, the Saikosaponina group and the Fluoxetine group, 15 rats in each group.Solitary and chronic mild unpredictability stimulation were used to copy the model of depression.From the second clay after modeling successfully, the Saikosaponina group and the Fluoxetine group were given intragastrical administration.On the first, seventh, fourteenth and twenty-first day of the experiment, sugar water consumption experiment was used to detect the behavioral change of the rats.Real-time PCR was used for the quantitative analysis of JNK, Bad mRNA. Result： The preference degree of sugar water in the the control group, the Saikosaponina group and the Fiuoxetine group was higher than that in the model group, the expression quantities of JNK, Bad mRNA in the the control group, the Saikosaponina group and the Fluoxetine group were lower than those in the model group, the differences were all statistically significant （ P〈0.01 ） .Conclusion： Saikosaponina can effectively control the depressive symptoms of rats. Its mechanism may be related to the decreasing of JNK, Bad protein expression in hippocampus.
出处
《中国医学创新》
CAS
2016年第3期28-30,共3页
Medical Innovation of China
基金
黑龙江省教育厅科学技术研究项目(12531784)
关键词
柴胡皂苷
海马
抑郁
凋亡信号调节激酶1
Saikosaponin
Hippocampus
Depression
Apoptosis sigual regulating kinase- 1
