摘要
目的研究低氧诱导因子-2α(HIF-2α)及其下游分解代谢因子(MMP3,MMP13,ADAMTs-4)在不同应力加载髁突软骨细胞中的表达变化情况。方法对培养到第3代的新生SD大鼠髁突软骨细胞采用5%的低氧培养箱培养。0.5 Hz下分别加载0,1 000,2 000,4 000单位压力,2 h后立即收集样本,台盼蓝染色,光镜下观察细胞形态并计数细胞数,计算死细胞率。采用westernblot及RT-PCR技术检测HIF-2α及其下游分解代谢因子(MMP3,MMP13,ADAMTs-4)的蛋白及基因表达。结果随着压力的增加,髁突软骨细胞死亡率显著上升(P<0.05)。HIF-2α及其下游分解代谢因子(MMP3,MMP13,ADAMTs-4)的表达相应增加(P<0.05)。结论超负荷压力激活HIF-2α,继而激活下游分解代谢因子可能是导致髁突软骨损伤的一个机制。
Objective To explore the expression of HIF-2α and its downstream catabolic factors （ MMP3, MMP13, ADAMTs-4） in the mandibular condyle chondrocytes （MCC） under different stress loadings. Methods The third generation of MCC were cultured in 5% hypoxia. The uniaxial pressure of 0, 1000,2000,4000 units strain （ust） were exerted on the MCC for 2 h. Then the specimens were collected and dyed by trypan blue staining. The morphology and number of cells were observed under a light microscope, and the cell mortality was calculated. Westernblot and RT-PCR were performed to detect the changes of expression of HIF-2α and its down- stream catabolic factors （ MMP3, MMP13, ADAMTs-4）. Results With the increase of pressure, the cell mortality increased （ P〈 0.05 ）. The expression of HIF-2α and its downstream catabolic factors （ MMP3, MMP13, ADAMTs-4） increased with the increase of pressure （ P〈0.05 ）. Conclusion The activation of HIF-2α and its downstream catabolic factors by overload pressure may be respon- sible for the damage of condyle chondrocytes.
出处
《口腔医学》
CAS
2016年第2期101-104,共4页
Stomatology
基金
浙江省自然科学基金项目(LY13H140001)
浙江省公益技术应用研究项目(2013C33154)
