摘要
为了能够从微生物多样性的角度解释湿地净化水体的原理,分析了下一代代测序技术——高通量测序技术的过程和原理,总结了高通量测序技术应用的优缺点和高通量测序技术在人工湿地微生物多样性中的应用。同时得出了高通量测序技术在人工湿地微生物群落的多样性和动态性中具有很大的应用价值,指出了高通量测序结果中处理庞大数据等问题。但是高通量测序技术具有其独特的优点,对人工湿地微生物多样性的研究具有很大的应用前景。
The paper aims to explain the theory of purifying the water body with wetland from the perspectiveof microbial diversity. The process and principle of the next-generation sequencing technology—high-throughput sequencing technology was analyzed, the advantages and disadvantages of high-throughputsequencing technology applications and the high-throughput sequencing technology application in wetlandmicrobial diversity were summarized in this paper. Meanwhile the broad application value of high-throughputsequencing technology in the diversity and dynamics of the microbial community of artificial wetland wasobtained, the high-throughput sequencing results in huge data processing and other issues were pointed out inthis paper. Because the high-throughput sequencing technology has unique advantages, it has a greatapplication prospect in the study of wetland microbial diversity.
出处
《中国农学通报》
2016年第5期10-15,共6页
Chinese Agricultural Science Bulletin
基金
中央级公益性科研院所基本科研业务费专项"复合湿地植物根系与基质微生物及浮游动植物群落动态结构的研究"(2015JBFM24)
"十二.五"国家科技支撑计划"长江下游池塘高效生态养殖技术集成与示范"(2012BAD25B07)
国家大宗淡水鱼类产业技术体系项目"国家大宗淡水鱼类产业技术体系"(nyeytx-46)
关键词
高通量测序技术
人工湿地
微生物多样性
应用前景
high-throughput sequencing technology
constructed wetlands
microbial diversity
application prospect
