摘要
目的:探讨经多巴胺预处理聚四氟乙烯人工血管对体外种植内皮细胞相对增值率影响,并评价其对内皮细胞的细胞相容性。方法:将内皮细胞与经多巴胺预处理后的人工血管片共培养48 h,观察细胞生长情况。并采用四唑盐比色法(MTT)测定不同浓度组材料浸提液(0、50%和100%)吸光度,并计算各组内皮细胞的相对增殖率和毒性分级。结果:经多巴胺预处理聚四氟乙烯人工血管对内皮细胞的贴壁和增殖无明显影响,且其培养上清液对内皮细胞毒性为0和1级。结论:经多巴胺处理后的聚四氟乙烯人工血管对内皮细胞无毒性,具有良好细胞相容性。
Objective： To detect the effect of relative proliferation rate which Polytetrafluoroethylene artificial blood vessels processed with dopamine on cultivatable endothelial cells in vitro,then evaluate the cytocompatibility of the endothelial cells. Methods： Endothelial cells were co-cultured with artificial blood vessels processed with dopamine for 48 hours. And tetrazolium salt colorimetry（MTT）was used to detect the absorbance of the extracted liquid of the material in different concentrations（0, 50% and 100%）, and their relative proliferation rate and cytotoxicity of endothelial cell was evaluated. Results： Polytetrafluoroethylene artificial blood vessels processed with dopamine imposed a less effect on adhesion and proliferation of endothelial cells, and the cytotoxicity of extracted liquid of material was 0 or 1 grade. Conclusion： Polytetrafluoroethylene artificial blood vessels processed with dopamine had no cytoxicity to endothelial cell and possessed a good cytocompatibility.
出处
《中国现代普通外科进展》
CAS
2016年第1期11-15,共5页
Chinese Journal of Current Advances in General Surgery
基金
上海市卫生局课题(00442)
关键词
聚四氟乙烯人工血管
多巴胺
细胞毒性
内皮细胞
Polytetrafluoroethylene artificial blood vessels
Dopamine
Cytoxicity
Endothelial cell
