摘要
黑麦1R染色体短臂(1RS)携带条锈病、叶锈病、秆锈病、白粉病和蚜虫等抗性基因。为了检测1RS上是否携带与赤霉病抗性相关的基因,本研究采用1RS特异标记Xscm9对192个来自不同国家的品种/系构成的小麦自然群体和1个重组自交系(RIL)群体(宁7840与Chokwang杂交的F_7群体,共184个系)进行了分子检测,并在2011 2013年采用单花滴注法于温室中进行赤霉病抗性鉴定。结果发现,自然群体中22个品种携带1RS,携带1RS的株系三季赤霉病平均病小穗率(PSS)均显著低于不携带1RS株系的PSS(P<0.01),表明1RS对降低病小穗率有显著作用。分子标记和基因组原位杂交(GISH)检测结果表明,宁7840携带1RS。通过对宁7840/Chokwang衍生的RIL群体进行赤霉病抗性鉴定和基因型分析,发现不论主效赤霉病抗性基因Fhb1(标记Xsts142)存在与否,携带1RS株系的PSS显著低于不携带1RS株系的PSS(P<0.01);方差分析表明,宁7840携带的Fhb1与1RS在赤霉病抗扩展性上无显著互作(P>0.05)。因此认为,黑麦1RS染色体很可能携带赤霉病扩展抗性相关基因,与Fhb1基因有累加效应。
The short arm of 1R chromosome （1RS） of rye carries resistant genes to stripe rust, leaf rust, stem rust, powdery mildew and aphids. To understand if 1RS also mediates resistance to wheat Fusarium head blight （FHB）, we genotyped a panel of 192 wheat accessions from diverse geographic regions and a population of recombinant inbred lines （RIL） consisting of 184 lines developed from the cross of Ning 7840 and Chokwang by 1RS-specific molecular marker Xscm9 and evaluated FHB severities in three consecutive seasons in greenhouses using single floret inoculation method. The results demonstrated that 22 of 192 accessions carried a T1BL·1RS translocation, and the mean FHB severity （PSS） of varieties carrying T1BL·1RS translocation was significantly lower than that of lines without the translocation across the three experiments （P 〈 0.05）, indicating 1RS may have a positive effect on reducing FHB severity. 1RS-specific marker Xscm9 and Genome in situ hybridization （GISH） showed Ning 7840 carries T1BL·1RS translocation. In the population of RILs, irrespective of Fhbl locus, the mean PSS of lines with T1BL·1RS translocation was significantly lower than that of those lines without T1BL·1RS. The effects of Fhbl and 1RS on FHB resistance were additive and the interactions between them were not significant （P = 0.48）. The results of this study suggested that IRS of rye most likely carries the genes resistant to FHB.
出处
《作物学报》
CAS
CSCD
北大核心
2016年第3期320-329,共10页
Acta Agronomica Sinica
基金
国家转基因生物新品种培育科技重大专项(2012ZX08009003-004)
国家自然科学基金项目(31171537)
江苏省高校优势学科建设工程项目(PAPD)资助~~
