摘要
目的研究旋毛虫对噁唑酮（OXZ）诱导的实验性小鼠肠炎模型的影响及其免疫作用机制。
方法观察感染旋毛虫和未感染旋毛虫小鼠于OXZ诱导肠炎后3 d及7 d不同指标的变化，包括小鼠生存率、疾病活动指数（DAI）、结肠大体损伤和病理损伤评分、炎症指标髓过氧化物酶（MPO）活性检测，结肠及脾脏细胞因子IFN-γ、IL-4和IL-10 mRNA及蛋白的表达量分析。
结果预先感染旋毛虫后诱导OXZ模型组小鼠在造模后3 d及7 d与单纯模型组相比，小鼠生存率、DAI、MPO活性稍有所改善，差异未见统计学意义（P〉0.05），结肠大体损伤和病理损伤虽未见减轻，也未见进一步加重。结肠及脾脏中IFN-γ和IL-10 mRNA及蛋白的表达量上调（P〈0.05）,而IL-4 mRNA及蛋白的表达量增加（P〈0.05）或未见明显改变。
结论感染旋毛虫既未减轻OXZ模型小鼠肠道的炎症反应，也没有按理论所设想的感染旋毛虫所产生的Th2型免疫应答会对OXZ诱导的Th2型炎症反应起叠加作用而加重病情。可能是旋毛虫针对该模型诱生出发挥不同剂量依赖性作用的IL-10。提示我们，需进一步寻找并除去旋毛虫诱导IL-10过量表达的相关可变因素，即可以发挥IL-10抗Th2型免疫炎症反应的优势，可能使旋毛虫对噁唑酮诱导的肠炎也产生良好的干预效果。
ObjectiveTo study the effects of Trichinella spiralis （T.spiralis） infection on mice with oxazolone （OXZ）-induced colitis and the possible immunologic mechanism.
MethodsFemale BALB/c mice at age 6-8 weeks were randomly divided into four groups （A to D）. Each mouse in groups B and D was infected with T. spiralis strains. Twenty-one days after T. spiralis infection, the mice in groups A and B were treated with 50% ethanol solution, while those in groups C and D were treated with OXZ to induce the murine model of colitis. Several parameters including survival rate, disease activity index （DAI）, macroscopic damage and histological score, myeloperoxidase （MPO） activity and the expression of cytokines （IFN-γ, IL-4 and IL-10） in colonic and splenic tissues at mRNA and protein levels were measured 3 and 7 days after modeling.
ResultsNo significant differences in the survival rate, DAI score, macroscopic damage score, histological score, MPO activity were observed between mice from groups C and D （P〉0.05）. Pre-exposing the mice to T. spiralis strains neither alleviated the mucosal damages nor aggravated the condition of colitis. Compared with group C, the expression of IFN-γ on the third day and the expression of IFN-γ, IL-4 and IL-10 on the seventh day at mRNA and protein levels in colon and spleen tissues were significantly increased in mice treated with T. spiralis and OXZ （P〈0.05）. The expression of IL-10 at transcriptional level in spleen tissues on the third day was higher than that of group C （P〈0.05）. The expression of IL-4 and IL-10 at protein level in colon tissues on the third day were significantly up-regulated as compared with those of group C （P〈0.05）.
ConclusionThe severity of OXZ-induced colitis in the murine model was neither alleviated nor aggravated by pre-exposing the mice to T. spiralis strains. High doses of IL-10 not only weakened the regulatory effects on Th2 responses, but also induced the production of proinflammatory cytokines, resultin
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2016年第1期34-41,共8页
Chinese Journal of Microbiology and Immunology
基金
基金项目：国家自然科学基金（NSFC30600276）
关键词
旋毛虫
炎症性肠病
作用机制
Trichinella spiralis
Inflammatory bowel disease
Mechanism
