摘要
本文应用ABC法检测了30例尖锐湿疣组织中凝集素的结合活性。所用凝集素探针包括ConA、PNA、RCA_1、SBA和UEA_1。正常鳞状表皮呈区域性分布,凝集素结合的组化定位一般在细胞表面和细胞间质,偶而位于胞浆内。在尖锐湿疣组织中棘细胞层被ConA深染,而被其他凝集素中等程度着色,这种染色强度较正常表皮染色有所降低。表皮凝集素结合的细胞化学分布可能表明表皮正常分层和角朊细胞分化的表达,而且这种典型结合形式的消失可能提示严重增生不良。
Histochemical detection of lectin binding was carried out using ABC method, in condyloma accuminata,The lectins used for demonstrating sugar resi- dues were: ConA, PNA, 12CA 1, SBA and UEA 1. Leetin binding in normal squmaous epithelium showed reginoal distribution patterns of keratinized, spinous and basal layer types. Histochemical localization of lectin binding was generally at cellular surface and in the intercellular substances and sometimes in the cytoplasm of normal epithelial cells. In condyloma specimans, spinous cells showed an intense ConA staining and moderate binding by other lectins, which was somewhat decreased when compared with that of the normal epithelium. The cytochemical distribution of epithelia^1 lectin binding may be indicative of the expression of nomal stratification and keratinocytic differentiation, and the disappearance of this typical epithelial pattern may suggest severe dysplasia.
出处
《中国皮肤性病学杂志》
CAS
北大核心
1991年第4期199-201,261-262,共3页
The Chinese Journal of Dermatovenereology
