摘要
目的 探讨先天性人巨细胞病毒（HCMV）感染对肝功能与乙型肝炎疫苗免疫效果的相互影响。方法 孕鼠于见栓3~5d腹腔注射HCMV悬液,其仔鼠作为先天性HCMV感染模型,随机分为先天性HCMV感染组（感染组）、更昔洛韦治疗组（治疗组）,以健康大鼠的仔鼠作为对照组。各组仔鼠分别于生后1、3、5周接种乙肝疫苗,并于3、5、7、11周心脏采血,采血后2h内应用全自动生化分析仪检测丙氨酸氨基转移酶（ALT）浓度,留取血清应用酶联免疫分析方法检测血清乙肝表面抗体（HBsAb）、透明质酸（HA）、层黏连蛋白（LN）、Ⅲ型前胶原（PCⅢ）和Ⅳ型胶原（ColⅣ）的浓度。结果3、5、7、11周时感染组ALT浓度分别为（66.31±6.62）、（55.14±8.03）、（55.27±6.50）、（49.85±8.06）IU/L,均高于治疗组与对照组,差异均有统计学意义（P〈0.05）;3周时治疗组仔鼠的ALT浓度高于对照组（P〈0.05）。在各时间点（3、5、7、11周）,感染组HBsAb滴度分别为（0.54±0.17）、（1.28±0.66）、（2.45±0.74）、（2.13±0.76）U/ml,均低于治疗组和对照组,差异均有统计学意义（P〈0.05）,治疗组HBsAb滴度低于对照组,差异有统计学意义（P〈0.05）。在各时间点（3、5、7、11周）,感染组仔鼠的HA、LN、PCⅢ和ColⅣ的浓度均显著高于治疗组与对照组,差异有统计学意义（P〈0.05）;3周、5周时治疗组HA、LN、PCⅢ检测结果高于对照组,异有统计学意义（P〈0.05）。结论 先天性人巨细胞病毒感染可造成肝损伤,影响肝功能,导致机体对乙肝疫苗免疫应答降低;及时治疗,有利于改善肝功能,增强乙肝疫苗的免疫效果。
Objective To explore the interaction of congenital human cytomegalovirus infection in liver function and im- mune response to hepatitis B vaccine. Methods Pregnant rats were intraperitoneally inoculated with HCMV suspension on 3-5 gestation days. The newborn rate were the model of congenital human cytomegalovirus infection and were divided into the ganciclovir treatment group and the infection group, while the healthy rats＇ offsprings were used as the control group. Offspring rats in both groups were inoculated with hepatitis B vaccine in the postnatal 1st, 3rd and 5th week and blood were taken from hearts separately in the 3rd, 5th,7th and l lth week. Alanine aminotransferase （ALT）density was detected by automatic biochemistry analyzer within two hours after the blood collection. Serum specimens were stored and hepatitis B surface antibody （HBsAb） ,hyaluronie acid （HA） ,laminin （LN）, HI procollagen（PCl]I ） and collagen IV （Col IV） concentration were detected by ELISA method. Results The ALT density of the infection group at the time points of 3rd,Sth,Tth and 11th week was （66.31±6.62）, （55.14±8, 03）,（55.27±6.50）,and 49.85±8.06） IU/L,which was higher than that in the ganciclovir treatment group and the control group（ P d0.05）. The ganciclovir treatment group＇s ALT density was higher than that in the control group at the time point of 3rd week （ P 〈0. 05）, At 3rd,Sth,7th and llth week,the titer of HBsAb in the infection group was（0.54±0.17）, （1.28±0.66） ,2.45±0.74） ,and （2.13±0.76） U/ml, which was lower than that in the control group and the ganciclovir treatment group respectively（ P 〈0.05）, while the ganciclovir treatment group was lower than that in the control group（ P 〈0.05）. At all time points（3rd,Sth,7th and 11th week）,the density of HA,LN,PCⅢ ,and Coliv in the infection group was higher than that in the ganciclovir treat- ment group and the control group. The density of HA,LN,and PCⅢ in the ganciclovir treatment g
出处
《预防医学论坛》
2016年第1期54-57,共4页
Preventive Medicine Tribune
关键词
人巨细胞病毒
先天性感染
肝功能
乙肝疫苗
免疫效果
Human cytomegalovirus
Congenital infection
Liver funetion
Hepatitis B vaceine
Immune effect
