摘要
研究南繁区棉花炭疽病菌的生物学特性为筛选其杀菌剂提供理论依据。从海南省南繁棉花区采集病样分离得到病原菌,对病原菌进行生物学特性研究。结果表明：棉花炭疽病菌（HNNC8）菌丝在PDA培养基上生长最好;菌丝生长和孢子萌发的最适温度分别为25和30℃;最适p H值分别为7～9和6～8;连续黑暗条件较适合菌丝生长;菌丝在以麦芽糖、阿拉伯糖为碳源的培养基上生长最好;在以蛋白胨为氮源培养基上生长最好;病菌分生孢子致死温度为55℃水浴处理5 min。温度过高或过低酸性环境都会影响菌丝生长和分生孢子的萌发,且不同的碳源、氮源亦会影响病菌菌丝的生长。
The paper was to study the biological characteristics of Colletotrichum gossypii in Hainan, and preliminarily screen its fungicides. The pathogen was isolated from the infected leaves of cotton collected from breeding in Hainan province, The biological characteristics of C. gossypii. The mycelium had the best growth on the medium with Potato Dextrose Agar. The optimum temperature for mycelial growth and spore germination were 25 and 30℃,and the optimum p H was 7 ～9 and 6 ～8, respectively. The continuous dark condition was more suitable for mycelial growth. The suitable carbon sources were maltose, L-arabinose, and the suitable nitrogen sources were peptone. The lethal temperature of spore was55 ℃ water bath for 5 min. Temperature too high or too low, acidic environment will affect the mycelial growth and spore germination, and different carbon sourceor nitrogen source, will also affect the growth of hypha.
出处
《热带农业科学》
2016年第1期51-57,共7页
Chinese Journal of Tropical Agriculture
基金
南繁区生物安全监测预警及控制关键技术研究与示范(No.201403075)
海南省自然科学基金(No.20153131)
国家农业产业技术体系建设(No.CARS-34-GW8)
农业部2013年热作农技推广与体系建设项目(No.13RZNJ-20)
关键词
棉花
棉花炭疽病菌
生物学特性
cotton
Colletotrichum gossypii Soutllw.
biological characteristics
