摘要
目的 探讨微小核糖核酸（microRNA，miRNA）-145在新疆维吾尔族和汉族妇女不同宫颈组织中的表达情况，及其与宫颈癌临床病理特征的关系。方法收集2010年11月至2013年2月住院手术治疗并经术后病理确诊的维族和汉族妇女不同宫颈石蜡组织标本150例，其中宫颈癌组织58例，宫颈上皮内瘤变（CIN）Ⅱ～Ⅲ级组织60例和正常宫颈组织32例。采用实时荧光定量逆转录-聚合酶链反应（qRT—PCR）方法检测不同宫颈病变中miR—NA-145的表达，分析其在不同宫颈病变之间及两民族妇女间表达的差异，以及miRNA-145表达与宫颈癌临床病理特征和人乳头瘤病毒（HPV）感染的关系。结果miRNA．145在宫颈癌组织中的表达均显著低于正常宫颈组织和CINⅡ-Ⅲ级组织（P均〈0．05），维、汉族妇女间表达差异无统计学意义（P〉0．05）。miRNA-145的表达与宫颈癌组织分化程度、FIGO分期、淋巴结转移及高危型HPV感染显著相关（P〈0．05或P〈0．01），与患者年龄、肿瘤直径、病理类型、肌层浸润均无相关性（P均〉0．05）。ROC曲线分析显示，miRNA-145区分宫颈癌组织分化程度和有无淋巴结转移时，曲线下面积（AUC）分别为0．786（灵敏度91．7％，特异度65．2％）和0．779（灵敏度77．8％，特异度61．5％）。结论miRNA-145在宫颈癌组织中呈低表达，无种族表达差异，可能作为抑癌基因参与宫颈癌的发生、发展，有望成为宫颈癌早期诊断、治疗及预后评估的新型肿瘤标记物。
Objective To explore the expression of micro ribonucleotide （microRNA, miRNA）-145 in different cervical tissues in Uyghur and Han women of Xinjiang and its association with clinicopathological characteristics of cervical carcinoma. Methods A total of 118 paraffin tissue specimens of different cervical tissues confirmed by postoperative pathological examination [ 58 cervical cancer tissues ,60 stage Ⅱ-Ⅲ cervical intraepithalial neoplasia（CIN） tissues ] from Uyghur and Han women,who underwent operation treatment between November 2010 and February 2013, and 32 normal cervical tissue specimens were collected. Real time fluorescence quantitative reverse transcription polymerase chain reaction （qRT-PCR） was used to detect miRNA-145 expression in cervical tissues. The differences of miRNA-145 expression in different cervical lesions and in two ethnic groups were observed. The associations of miRNA-145 expression with clinicopathological charac- teristics of cervical carcinoma and with infection of human papillomavirus （HPV） were analyzed. Results The expression level of miRNA-145 in cervical carcinoma tissues was statistically lower than those in normal cervical tissue and stage Ⅱ - Ⅲ CIN tissues （ all P 〈 0.05 ）, while there was no ethnic difference （P 〉 0. 05 ）. The expression of miRNA-145 was closed correlated with the differentiation degrees of cervical carcinoma tissue, international federation of obstetrics and gynecology （FIGO） staging, lymph node metastasis and the infection of high-risk type HPV （P 〈 0.05 or P 〈 0.01 ）, while was not significantly correlated with patients＇ age, tumor diameter, pathologic type and muscle layer infiltration （all P 〉 0. 05 ）. The receiver operating characteristic curve （ROC）analysis showed that when using miRNA-145 for distinguishing cervical cancer tissue differentiation degree and judging whether there was lymph node metastasis, the areas under the ROC curve （AUC） were 0. 786 （ sensitivity was 91.7%, specificity w
出处
《中国临床研究》
CAS
2016年第2期173-177,共5页
Chinese Journal of Clinical Research
基金
国家自然科学基金资助项目(81160278)
关键词
微小核糖核酸-145
宫颈癌
宫颈上皮内瘤变
临床特征
人乳头瘤病毒
ROC曲线
维吾尔族
汉族
Micro ribonucleotide-145
Cervical cancer
Cervical intraepithelial neoplasia
Clinical characteristics
Human papillomavirus
Receiver operating characteristic curve
Uyghur population
Han population
