摘要
本文作者通过水热法合成了中空SiO_2-SH纳米微球,然后直接用其吸附Ni^(2+),从而形成SiO_2-SH-Ni^(2+)复合材料,以此材料为载体,可以将以组氨酸为标签的(His-tagged)的融合蛋白直接从细胞裂解液中进行分离纯化,实验结果表明,该微球适合于His-tagged融合蛋白的分离纯化.
Hollow SiO2-SH nanospheres were synthesized through the hytrodthermal method,then these microspheres were used to adsorb directly Ni^2＋ions to form SiO2-SH-Ni ^2＋composite materials.In the end,the His-tagged fusion proteins can be separated from the mixture proteins by the SiO2-SH-Ni^2＋ composite materials.The experimental results show that it is suitable for these prepared microspheres to separate the His-tagged proteins.
出处
《化学研究》
CAS
2016年第1期112-115,共4页
Chemical Research
基金
国家自然科学基金资助项目(21271062)
河南省教育厅科学技术重点研究项目(14B150003)
