摘要
以纤维素热解产物内醚糖为碳源筛选到4株高效利用内醚糖的微生物,经ITS测序和菌落形态特征鉴定,4株菌分别为嗜松青霉(Penicillium pinophilum)、桔青霉(Penicillium citrinum)、冠突散囊菌(Eurotium cristatum)和红假单胞菌(Rhodopseudomonas sp)。对各菌株利用内醚糖的研究结果表明,嗜松青霉和桔青霉5 d内对2%内醚糖的利用率为100%,冠突散囊菌7 d内对2%内醚糖的利用率为100%,红假单胞菌在10 d对2%内醚糖的利用率为52%。将4株菌作用于热解液发现,红假单胞菌能在未经预处理的热解液中生长,14 d后对热解液中内醚糖的利用率为69%,另外3株菌能在经5%活性炭+Ca(OH)2预处理后的热解液中生长。对预处理后热解液成分的分析表明,预处理对热解液成分有显著影响,其中六甲基环三氧硅烷、1,2-环戊二酮、5-甲基糠醛、3-甲基-1,2-环戊二酮和羟甲基呋喃酮的去除率达100%,内醚糖的损失为27.1%。
Four efficient levoglucosan-assimiliating microorganisms were screened using levoglucosan from cellulosis pyrolysis as carbon source, and they were identified as Penicillium pinophilum, Penicillium citrinum, Eurotium cristatum and Rhodopseudomonas sp based on morphology and ITS sequence analysis. The results show that the 2%-levoglucosan utilization efficiency of P. pinophilum, P. citrinum（4 d） and E. cristatum（7 d） are 100%, and that of Rhodopseudomonas sp is 52%（10 d）. Rhodopseudomonas sp can be cultured in untreated pyrolysis liquids with utilization efficiency of 69%（14 d）, while Penicillium pinophilum, Penicillium citrinum and Eurotium cristatum can be cultured in pretreated pyrolysis liquids（treated by 5% activated carbon＋Ca（OH）2）. The pretreated pyrolysis liquids were analyzed and the results show that pretreatment has obvious influence on the pyrolysis liquid. Hexamethylcyclotrisiloxane,1,2-Cyclopentanedione,5-methylfurfural, 3-methyl-1,2-Cyclopentanedione and hydroxymethylfuraneol are fully removed, and the loss rate of levoglucosan is 27.1%.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2016年第1期127-132,共6页
Journal of Chemical Engineering of Chinese Universities
基金
江苏省高校自然科学研究重大项目(15KJA210002)
江苏省“六大人才高峰”人才培养项目(NY-011-2015)
关键词
内醚糖
热解液
嗜松青霉
桔青霉
红假单胞菌
levoglucosan
pyrolysis liquid
Penicillium pinophilum
Penicillium citrinum
Rhodopseudomonas sp
