摘要
目的 探究绿茶多酚对动脉粥样硬化抑制作用的效能及其机制,为冠心病的防治提供理论和实验依据.方法 选取1.5～2.0kg约2月龄的纯种健康雄性新西兰大白兔10只,随机分为3组：正常对照组：饲普通颗粒饲料（糖、蛋白质及脂肪各占总热量的62％、17％及21％）;高脂饲料模型组：饲高脂饲料（7.5％蛋黄粉＋1％胆固醇＋3％猪油＋88.5％普通颗粒饲料）,蛋黄粉开始足量,胆固醇和猪油逐次增加（＋0.5％/次）,观察兔子无腹泻,进食量可则加,约3天左右加一次,直至目标剂量（胆固醇6％,猪油4％）;高脂饲料绿茶多酚组：饲料同高脂饲料模型组＋绿茶多酚100 mg·kg^-1·d^-1.均单笼喂养,常规饮水,每天每只进食总量控制在100～120g,连续喂养12周.实验末,经耳缘静脉取血,测定血清总胆固醇（TC）、甘油三脂（TG）、低密度脂蛋白胆固醇（LDL-C）及高密度脂蛋白胆固醇（HDL-C）水平;超声检查家兔髂外动脉及其远端血管情况;取主动脉组织,进行AS斑块面积及HE、MASSON染色检测.结果 高脂饲料模型组家兔主动脉内膜充满泡沫细胞,壁上可见斑块;高脂饲料茶多酚组家兔主动脉壁脂质沉积相对较轻.结论 绿茶多酚可降低高脂饲料诱导的动脉粥样硬动物模型的血脂水平,缓解血管脂质性变和纤维性变.
Objective To. explore the effectiveness of green tea polyphenols on inhibition of atherosclerosis and possible mechanisms, for the prevention and treatment of coronary heart disease （CHD） to provide theoretical and experi- mental basis. Methods 10 healthy male New Zealand white rabbit （1.5-2.0 kg, 2 months） were randomly divided into Normal control group with ordinary granulated feed （sugar, protein and fat were 62%, 17% and 62% of total calories）, High fat feed model group with high fat forage egg yolk powder （7.5 % egg yolk powder ＋ 1% lard ＋3% cholesterol, 88.5 % ordinary granulated feed） and High-fat feed green tea polyphenols groups with high fat feedstuff model group ＋ green tea polyphenol, mg·kg^-1·d^-1. At the end of the experiment, the ear vein blood, serum total cholesterol （TC）, triglycerides （TG）, low density lipoprotein cholesterol （LDL - C） and high-density lipoprotein cholesterol （HDL - C） level were measured. The rabbit iliac artery and its distal vessels were examined with ultrasound. The aorta tissue was stained with HE and MASSON. Results The aortic intima of high fat feed model group was full of foam cells. There were visible patches in the wails. The aortic lipid deposition of high-fat feed tea polyphenols group is relatively light. Conclusion Green tea polyphenols can reduce atherosclerosis induced by high-fat feed and relieve blood lipid change and fibrous.
出处
《西部医学》
2016年第2期177-180,共4页
Medical Journal of West China
基金
四川省卫生厅科研课题(120472)
关键词
绿茶多酚
动脉粥样硬化
纤维素
Green tea polyphenols
Atherosclerosis
Cellulose
