摘要
目的分析献血人群中HBV DNA定量检测结果与乙肝两对半模式的关联。方法对于献血者血液筛查出来的HBs Ag阳性者进行乙肝两对半检测、HBV DNA荧光定量PCR检测,对于荧光定量PCR高于1000 IU/ml的进行DNA测序。结果所测样本存在9种HBV-M模式,各种模式都有一定比率的DNA活动性复制,测序标本均为HBV B型。结论献血人群的乙肝病毒携带者中存在较高的病毒活动性复制,应予以重视和进一步研究,HBV基因型全部为B型,可能是本地献血人群的分布特点。
Objective To analyze the HBV-DNA quantitative test results and serum HBV markers in blood donors. Methods The positive HBsAg was detected as HBV markers in blood donors and HBV-DNA was tested by fluorescent quantitative-PCR, and samples over 1 000 IU/ml was subjected to DNA sequencing. Results 9 HBV-M modes in the measured samples were noted and each of the modes presented the active DNA replication in a certain degree. Conclusion There are a higher viral replication activity in HBV carriers in blood donors, to which more attention should be paid. The genotype B of HBV might be the endemic character in the local blood donors.
出处
《临床输血与检验》
CAS
2016年第1期59-61,共3页
Journal of Clinical Transfusion and Laboratory Medicine
