摘要
目的:观察应激对反流性食管炎模型大鼠食管下段p H值及黏膜血流的影响,探讨心理因素在反流性食管炎发生、发展中的作用。方法:雄性Wistar大鼠,随机分为对照组、应激组、模型组、应激-模型组,每组15只。采用改良部分贲门肌切开术联合外置幽门部分结扎术制备大鼠反流性食管炎模型。造模后7 d,应激组和模型-应激组用水浸束缚法行应激实验。实验结束后,各组大鼠麻醉、剖腹,检测各组大鼠食管下段p H值、黏膜血流。结果:与对照组比较,应激组大鼠食管下段p H值(3.21±0.32)、黏膜血流[(38.62±6.11)BPU]均下降(P<0.01),模型组大鼠食管下段p H值(2.23±0.27)、黏膜血流[(30.16±6.79)BPU]均下降(P<0.01);与模型组比较,应激-模型组大鼠食管下段p H值(2.01±0.19)、黏膜血流[(14.41±7.04)BPU]均下降(P<0.01)。结论:应激可加重反流性食管炎模型大鼠食管黏膜缺血、降低食管下段p H值。
ObjectiveTo observe the influence of pH values and blood flow of lower esophageal in refluxEsophagitis model rat by stress, and to explore the effect of esophageal by psychological factor.MethodsPre-operative rats were randomly divided into 4 groups, control group, stress group, model group and stress plus mod-eling group, 15 rats in each group. Reflux Esophagitis rat model was established by the method of improved partcardiac muscle incision and part outlay-pyloric ligation. 7 days later, stress group and stress plus modelinggroup were induced by water immersion and immobilization. And then, the pH values of distal esophagus, theesophageal mucosal blood flow were compared.ResultsCompare with control group, the stress group lead tothe pH values（3.21±0.32）and the blood flow[（38.62±6.11）BPU]down（P〈0.01）, the model group lead to thepH values（2.23±0.27）and the blood flow[（30.16±6.79）BPU]down（P〈0.01）; compare with model group, thestress plus model group lead to the pH values（2.01±0.19）and the blood flow[（14.41±7.04）BPU] down（P〈0.01）.ConclusionThe stress could aggravate ischemia of esophageal mucosa and make pH values and theblood flow down.
出处
《中国中西医结合外科杂志》
CAS
2016年第1期42-44,共3页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
基金
天津市卫生局科技基金(2011KZ50)
关键词
反流性食管炎
水浸束缚
黏膜血流
PH值
大鼠
Reflux Esophagitis
water immersion and immobilization
esophageal mucosal blood flow
pH values
rat
