摘要
目的 建立人脑胶质瘤SHG-44裸鼠皮下模型并了解SHG-44人脑胶质瘤裸鼠体内生长特性.方法 将雄性4周龄裸鼠按随机数表法分为高密度细胞悬液接种组（n=10）,低密度细胞悬液接种组（n=10）,肿瘤组织块接种组（n=10）及生理盐水注射的空白对照组（n=10）.将对数期生长的SHG-44人脑胶质瘤细胞悬液注射于裸鼠腋窝后外侧皮下,待肿瘤块形成后,切取肿瘤组织,并将肿瘤组织剪切成1 mm3组织块,重新接种于新的裸鼠腋窝后外侧皮下.接种后每天观察肿瘤组织生长情况并每5d记录肿瘤长径及短径,并与高低细胞悬液组对比,接种后60 d统一处死存活鼠,切取肿瘤组织行病理学及免疫组化检测.结果 肿瘤细胞悬液接种裸鼠,潜伏期较长、组织生长缓慢且成瘤率较低.瘤组织块接种后第20天左右肿瘤体积[（41.51±6.42） mm3]开始明显增大,增长迅速,形态良好,第50天组织块接种肿瘤体积[（565.69±123.36） mm3]较细胞悬液高密度组细胞悬液接种[（203.85± 104.63） mm3]、低密度细胞悬液接种[（153.02±31.76） mm3]明显增大,差异有统计学意义（均P＜O.05）.光镜下细胞悬液组及组织块组瘤体边界清晰,无明显浸润,瘤内血管丰富,免疫组化GFAP及S-100染色见大量阳性细胞.结论 以细胞悬液制备荷瘤鼠肿瘤为原材料,将瘤组织块接种建立人脑胶质瘤SHG-44裸鼠皮下模型,潜伏期短、生长速度快.
Objective To establish nude mouse model with human brain glioma SHG-44 and understand its growing characteristics in vivo.Methods The 4-week-old male mice were randomly divided into high density cell suspension inoculation group（n=10）,low density cell suspension group（n=10）,the tumor tissue mass vaccination group（n=10）and the blank control group with normal saline injection（n=10）.The SHG-44 human brain glioma cell suspension was injected into the subcutaneous of the nude mice＇ s armpit.The tumor tissue was cut into 1 mm3 after tumor tissue growth and formation,and re-inoculated into the subcutaneous of the new nude mice＇ s armpits.Apart from daily observation,the long and short diameters of tumor were recorded every 5 days after graft.All the mice were sacrificed at 60 days and the tumor tissues were harvested for pathological examination.Results With a longer incubation period and slower growth rate,the tumor formation rate in high density cell suspension inoculation group and low density cell suspension group was lower compared with that in the tumor tissue mass vaccination group.Around day 20,grafted tumor appeared remarkably big（（41.51 ±6.42）mm3） with good morphology.On day 50,the tumor derived from group the tumor tissue mass vaccination group（（565.69± 123.36）mm3） showed a bigger size in comparison with that from high density cell suspension inoculation group（（203.85±104.63） mm3） and low density cell suspension group （（153.02± 31.76） mm3,all P＜0.05）.The tumors in three groups were well defined with a rich vascularity and no apparent invasion was observed.The positive expression of GFAP and S-100 in a large body of tumor cells was observed under optical microscope.Conclusion With a shorter incubation period and faster growth,the mouse tumor models established with tissue pieces from the tumor-bearing mice are much better compared to those with cell suspension.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2016年第1期24-28,共5页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金（81560409）
贵州省科学技术基金（[2012]2051号）
