摘要
【目的】研究连翘酯苷A体外抗鸡传染性支气管炎病毒(IBV)作用及机理。【方法】MTT检测细胞活力,连翘酯苷A作用于IBV感染细胞36h,Quantitative Real-time PCR检测细胞内IBV N基因变化、细胞内受体(MDA5,LGP2,NLRC5)和抗病毒基因(IRF7,IFN-α,IFN-β)mRNA表达变化。【结果】连翘酯苷A作用于IBV感染细胞36h,可显著抑制细胞内IBV复制,同时显著提高细胞内受体(MDA5,LGP2,NLRC5)和抗病毒基因(IRF7,IFN-α,IFN-β)mRNA表达。【结论】连翘酯苷A具有抗IBV作用,且能激活IBV感染细胞内受体(MDA5,LGP2,NLRC5)和抗病毒基因(IRF7,IFN-α,IFN-β)。
【Objective】The aim of this study was to evaluate the anti-infectious bronchitis virus(IBV)activity of forsythoside A and to investigate the mechanism involved in its antiviral action.【Methods】Cell viability was measured by MTT.The IBV-infected HD11 cells were treated with forsythoside A at different concentration(0-20μmol/L)for 36 hours,then Quantitative Real-time PCR was used to determine IBV N gene,intracellular receptors(MDA,LGP2 and NLRC5)and antiviral gene(IRF7,IFN-αand IFN-β)mRNA expression.【Results】Forsythoside A significantly inhibited IBV infection and up-regulated intracellular receptors(MDA,LGP2 and NLRC5)and antiviral gene(IRF7,IFN-αand IFN-β)mRNA levels.【Conclusion】Forsythoside A exerts anti-infectious bronchitis virus activity and activates intracellular receptors(MDA,LGP2 and NLRC5)and antiviral gene(IRF7,IFN-αand IFN-β)in IBV-infected cells.
出处
《北京农学院学报》
2017年第1期37-42,共6页
Journal of Beijing University of Agriculture
基金
国家自然基金项目(31372485)
