摘要
为准确定量肉及肉制品中羊源性成分的含量,建立了微滴数字PCR定量检测系统。结果显示在一定范围内羊肉质量与DNA含量、DNA含量与DNA拷贝数之间均呈现明显的线性关系,选择DNA含量作为中间换算值,计算出羊肉的质量与拷贝数之间的关系为M_羊=0.03C+0.69。应用建立的微滴数字PCR定量检测方法对已知质量分数的羊肉模拟混合样品进行检测,发现无论在两两混合还是多肉样混合的情况下,其含量偏差最大为1.52%,具有较强的抗干扰能力。通过对市售样品的检测,显示该方法能够准确检测出不同样品中羊肉的含量,并发现可能存在掺假现象,说明该检测方法具有良好的市场应用前景。本实验建立的微滴数字PCR定量方法在肉及肉制品中羊源性成分检测方面具有较大的应用潜力,为肉制品日常检测及是否掺假提供有力的科学依据。
A quantitative method based on the droplet digital polymerase chain reaction（dd PCR） technique was developed to determine the weight of ovine in meat products. By using the dd PCR method,the relationships between the meat weight and DNA weight and between the DNA weight and DNA copy number were both close to linear within the dynamic range. The DNA weight was utilized as an intermediate value to establish the following formulae for calculating the original meat weight from the DNA copy number：Movine=0.03C＋0.69. By examining the mixed meat samples of known composition,the final quantitative results were similar to the true meat weights,and the maximum content deviation was 1.52%. Analysis of commercial samples showed that dd PCR quantification system could determine the proportion and quantification of ovine and had a good practicability. Quantitative analysis indicated that dd PCR was highly precise in quantifying ovine in meat products and had the potential to be used in routine analysis and quality meat adulteration of various species.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第4期73-76,共4页
Science and Technology of Food Industry
基金
国家质检总局科技计划项目(2014IK089)
关键词
微滴数字PCR
定量
羊源成分
掺杂
droplet digital polymerase chain reaction
quantification
ovine products
adulteration
