摘要
采用水蒸气蒸馏法提取黄檗果实精油,利用气相色谱-质谱联用技术(GC-MS)对其成分进行分离鉴定;通过DPPH自由基清除作用和β-胡萝卜素漂白法检测精油的抗氧化活性,应用磺酰罗丹明B(SRB)比色法检测精油对人宫颈癌细胞He La、人肺癌细胞A-549和人胃癌细胞BGC-823这3种肿瘤细胞株的细胞毒活性。经GC-MS分析,从黄檗精油中共鉴定出39个成分,主要有β-蒎烯、β-氧化石竹烯、3-亚甲基-4-异丙烯基环己醇、西松烯等。精油具有一定抗氧化活性,对肿瘤细胞株A-549和BGC-823的细胞毒活性不显著,但对He La细胞具有较好的细胞毒活性。
The essential oil of the fruit of Phellodendron amurense was extracted with steam distillation and its constituents were analyzed by gas chromatography-mass spectrometry（ GC-MS）. The antioxidant activities were screened by DPPH radical-scavenging and β-carotene bleaching assays. The cytotoxic activity of the essential oil against He La,A- 549 and BGC- 823 human tumor cell lines was investigated by Sulforhodamine B（ SRB） method. GC-MS analysis identified 39 components of the essential oil. The major constituents were β-pinene, β-caryophyllene oxide, 3-methylene-4-isopropenylcyclohexanol and cembrene. The essential oil showed weak antioxidant activity in the DPPH and β-carotene tests. The essential oil showed weak cytotoxic activity on A-549 and BGC-823. However,it was worth to note that the essential oil showed high cytotoxicity against He La cancer cell lines.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2016年第2期147-151,共5页
Fine Chemicals
基金
东北林业大学大学生科研训练项目
中央高校基本科研业务费专项资金项目(2572015CB18
2572015CB17)
黑龙江省博士后科研启动金(LBH-Q15009)
林业公益性行业科研专项经费项目(20140470102)~~
关键词
黄檗果实
精油
化学成分
抗氧化活性
细胞毒活性
中药现代化技术
Phellodendron amurense fruits
essential oil
chemical composition
antioxidant activity
cytotoxic activity
modernization technology of traditional Chinese medicines
