摘要
目的探讨抗病毒治疗对慢性HBV感染者T淋巴细胞趋化因子受体及血清趋化因子表达水平的影响。方法选取成都市公共卫生临床医疗中心于2012年5月至2012年12月收治的肝炎患者100例,其中男68例,女32例,年龄34~72岁,平均年龄为（54.6±4.3）岁,分为慢性乙型肝炎（CHB）组60例,重型慢性肝炎组40例。选取同期于本院接受健康检查的正常人群20例为健康对照组。检测干扰素治疗前、治疗后4周、12周、24周、48周时外周血T淋巴细胞趋化因子受体（CXCR3、CCR6）及血清趋化因子水平（CXCL9、CXCL11、CCL20）、HBs Ag、HBV DNA和ALT水平。结果 CHB组、慢性重型肝炎组及健康对照组间CXCR3、CCR6、CXCL9、CXCL11、CCL20表达差异有统计学意义（F值分别为2.067、2.914、4.791、2.582、3.802,P值分别为0.043、0.031、0.011、0.034、0.024）。干扰素治疗12周、24周、48周后CHB患者血清HBV DNA、HBs Ag、ALT均有不同程度下降,T细胞趋化因子受体及血清趋化因子的表达下调,与治疗前比较差异有统计学意义（P均〈0.05）,但治疗4周时与治疗前比较差异无显著统计学意义（t值分别为1.23、0.74、1.64,P值分别为0.063、0.481、0.051）。结论CXCR3、CCR6及其配体（CXCL9、CXCL11、CCL20）参与了HBV感染后肝脏损伤,干扰素治疗可以影响CXCR3、CCR6及其配体（CXCL9、CXCL11、CCL20）的表达水平。
Objective To investigate the influence of antiviral therapy on the expression of T lymphocytes chemokine receptors and serum chemokines in patients with chronic hepatitis B. Methods Total of 100 patients with hepatitis in Chengdu Public Health Clinical Medical Center from May 2012 to December 2012 were selected and divided into CHB group and chronic severe hepatitis group. Among the patients, 68 cases were male and 32 cases were female, the average age was（54.6 ± 4.3） years（34~72 years） old. The serum HBV DNA, HBs Ag, ALT, chemokines（CXCL9, CXCL11, CCL20） and T lymphocytes chemokine receptors（CXCR3, CCR6） were detected before and after the interferon treatment for 4 weeks, 12 weeks, 24 weeks, and 48 weeks. Total of 20 healthy persons who accepted health examination in our hospital were selected as the healthy control. Results Among the CHB group, chronic severe hepatitis group and the healthy control group, the expression of T lymphocytes chemokine rceptors（CXCR3, CCR6） and serum chemokines（CXCL11, CXCL9, CCL20） had significant difference（F = 2.067, 2.914, 4.791, 2.582, 3.802; P = 0.043, 0.031, 0.011, 0.034, 0.024, respectively）. The serum HBV DNA, HBs Ag, ALT, chemokines and T lymphocytes chemokine receptors had declined significantly after the interferon treatment for 12 weeks, 24 weeks, 48 weeks（P〈0.05）, and for 4 weeks, there was no statistical difference（t = 1.23, 0.74, 1.64; P = 0.063, 0.481, 0.051, respectively）. Conclusions CXCR3, CCR6 and the ligands（CXCL9, CXCL11, CCL20） were involved in HBV-related liver damage. Interferon therapy can influence the expression levels of CXCR3, CCR6 and the ligands（CXCL9, CXCL11, CCL20）.
出处
《中国肝脏病杂志（电子版）》
CAS
2015年第4期42-46,共5页
Chinese Journal of Liver Diseases：Electronic Version
基金
四川省卫计委课题(120559)
