摘要
目的:研究乳安凝胶膏剂对大鼠乳腺增生组织中血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)与增殖细胞核抗原(Proliferating Cell Nuclear Antigen,PCNA)表达的影响。方法:56只雌性大鼠按随机数字表法分为正常对照组、模型组、赋形剂对照组、乳增宁组、乳安凝胶膏4.16 g/kg、2.08 g/kg、1.04 g/kg剂量组,共7组,采用注射苯甲酸雌二醇、黄体酮造模,给药30 d。免疫组化法测定各组大鼠乳腺组织PCNA和VEGF的表达。结果:乳安凝胶膏剂4.16 g/kg、2.08 g/kg组能降低大鼠乳腺PCNA和VEGF的表达,与模型组比较,差异均有统计学意义(P<0.05,0.01)。结论:乳安凝胶膏剂可通过抑制其乳腺血管内皮生长因子和增殖细胞核抗原的表达活性,达到对抗大鼠乳腺增生的目的。
Objective： To observe the mechanism of Ru＇an Ningjiao ointmentm on tissue of rat vascular endothelial growth factor（ PCNA） and the influence of PCNA（ VEGF） expression in rats with hyperplasia of mammary gland. Methods： A total of 56 female unpregnant rats were randomly divided into 7 groups： normal control group,model group,excipient control group,Ruzengning group,Ru＇an Ningjiao ointment 4. 16 g / kg,2. 08 g / kg,1. 04 g / kg dose groups. Models were produced by injecting estradiol benzoate and progesterone for 30 days. Use immunohistochemical method to determine the expression of PCNA and VEGF in rats with breast hyperplasia. Results： Ru＇an Ningjiao ointment 4. 16 g / kg,2. 08 g / kg group can reduce the expression of PCNA and VEGF,and compared with model group,the difference had statistical significance（ P〈0. 05,0. 01）. Conclusion： Ru＇an Ningjiao ointment can inhibit the mammary gland vascular endothelial growth factor and the expression of cell activity,and thus to control hyperplasia of mammary gland.
出处
《世界中医药》
CAS
2015年第12期1922-1925,共4页
World Chinese Medicine
基金
江苏省中医药管理局研究项目(编号:LZ09044)
