摘要
β-防御素作为抗菌肽的一种,对机体防病抗病具有重要的意义。为研究牛β-防御素在牛各组织脏器的分布,本试验通过制备兔抗牛β-防御素(BNBD)4和5的多克隆抗体,利用免疫组化的方法观察健康牛肺、脾、肝、肾组织以及渗出性炎症、结核性肉芽肿、增生性炎症情况下牛肺组织中两种防御素的表达。结果发现牛β-防御素4、5主要分布于各组织中的淋巴细胞、巨噬细胞胞浆及其周围分泌物中,以及肺泡上皮细胞胞浆及其分泌物中;同健康组织相比,病变组织中BNBD-4表达量升高,而BNBD-5表达量降低。本研究为探讨β-防御素的功能奠定了基础。
β-defensin, an antibacterial peptide, has important role in the prevention and treatment of disease. To investigate the distribution of bovine β-defensin （BNBD） , BNBD-4 and BNBD-5 was detected in normal tissues （lung, kidney and spleen） and pathologic lung tissues by immunohistochemical method using rabbit polyclonal antibodies. The results showed that in normal tissues, BNBD-4 and BNBD- 5 were mainly located in the cytoplasm of lymphocytes, macrophages and alveolar epithelial cells, and the secretion around these cells. Compared with the normal tissues, the expression level of BNBD- 4 was higher but the BNBD-5 level was lower in pathological lung tissues.
出处
《畜牧与兽医》
北大核心
2015年第12期25-30,共6页
Animal Husbandry & Veterinary Medicine
基金
科技部863计划项目(2012AA101302)
国家大学生创新训练项目
关键词
牛
Β-防御素
健康组织
病变组织
分布
bovine
β- defensin
normal tissues
pathological tissues
distribution
