摘要
目的：分析幽门螺杆菌细胞毒素相关蛋白（cagA）、空泡毒素相关蛋白（vacA）基因分型在2型糖尿病（T2DM）患者中的表达,探讨其对T2DM的影响. 方法：选择2012年3月～2014年8月来我院就诊的2型糖尿病患者100例作为研究对象,设为T2DM组,选择同时间来院就诊的非糖尿病患者100例作为对照组,分别采用血清抗 Hp-IgG 检测、14C尿素酶呼气实验和快速尿素酶试验进行 Hp感染的确定,采用测序的方法对Hp进行基因分型,筛查Hp感染的cagA、vacA基因分型. 结果：T2DM组中HP感染者63例,占63%,未感染者37例,占37%;对照组感染者27例,占27%;糖尿病组患者感染HP和未感染HP的血糖差异无同学意义（ P＞0.05）,但均明显高于对照组（P＜0.05）;高密度血脂比较,对照组明显高于糖尿病组,差异有统计学意义（P＜0 .05 ） ,糖尿病组中感染HP和未感染HP者差异无统计学意义,低密度血脂糖尿病组感染HP者明显高于对照组和未感染HP糖尿病者,差异有统计学意义（P＜0.05）,对照组感染HP者中cagA占比为27.03%,T2DM组感染HP者中占比为：76.19%,T2DM组感染HP者明显高于对照组患者（P＜0.05）,对照组感染HP者中vacA占比为45.95%,T2DM组感染HP者中vacA占比为76. 47%,T2DM组感染HP者明显高于对照组患者（P＜0.05）. 结论：2型糖尿病患者易感染幽门螺杆菌,且HP的两个毒力基因ca-gA、vacA的存在会加重T2DM的病情并会出现后期的胃炎等并发症。
Objective：To analyze the Helicobacter pylori cytotoxin associated gene （cagA）, vacuolar associated protein toxin （vacA） genotyping in type 2 diabetes mellitus （T2DM） patients express, explore its impact on T2DM.Methods：March 2012 -2014 in August to our hospital 100 patients with type 2 diabetes as an object of study , to T2DM group, select the same time to the hospital for treatment of non-diabetic patients with 100 cases as the control group, respectively serum anti -Hp-IgG detection, 14C urease breath test and rapid urease tests Hp infection were determined using sequencing methods for Hp genotyping screening Hp infection cagA , vacA genotyping . Results：T2DM group HP infected group accounted for 63 cases, accounting （63%）, uninfected group accounted for 37 cases, account-ing （37%）;infection control group accounted for 27 cases （27%）;, diabetes group patients infected with HP and HP glycemic differ-ences uninfected students no significance （P〉0.05）, but were significantly higher （P 〈0.05）;high-density lipids compared to the control group was significantly higher than that of the diabetic group , the difference was statistically significant （P 〈0.05）, diabetes group infected and uninfected HP HP were no significant differences , low-density lipids in diabetic group was significantly higher than those infected with HP and HP diabetes uninfected , the difference was statistically significant （P 〈0.05） in the control group who cagA HP infection accounted for 27.03%, T2DM group of persons infected with HP accounted for：76.19%, T2DM group were significantly higher in patients with HP infection （P 〈0.05）, HP infection control group vacA who accounted for 45.95%, T2DM group of infected persons vacA HP accounted for 76.47%, T2DM group were significantly higher in patients with HP infection （P 〈0.05）.Conclusion：Patients with type 2 diabetes susceptibility of H .pylori, and the two virulence genes cagA HP＇s, vacA presence of the disease would in-creas
出处
《中国伤残医学》
2016年第2期9-11,共3页
Chinese Journal of Trauma and Disability Medicine
基金
佛山市科技局,2014年佛山市医学类科技攻关项目
