摘要
目的：验证同型半胱氨酸（Hcy）是否具有促进大鼠血管平滑肌细胞（VSMCs）去分化的作用。方法：SD大鼠主动脉VSMCs原代细胞培养鉴定，取4～7代细胞用于实验。VSMCs分为对照组、100μmol／LHcy干预组、500pmol／LHcy干预组、1000μmol／LHcy干预组共4组。MTT法测各组VSMCs的增殖情况；划痕法和Transwell法检测各组VSMCs的迁移情况；ICC法检测各组VSMCs的细胞形态；Westernblot法检测各组VSMCs中SM-actin、SM．MHC、Calponin、骨桥蛋白（OPN）的表达情况。结果：相比于对照组，Hcy组VSMCs增殖迁移增加；细胞形态变圆；SM．MCH和Calponin表达减少（P〈0．01）；OPN表达增加（P〈0．01），Hcy的这一作用与浓度呈正相关。结论：Hcy可以促进VSMCs去分化，这可能是其促进VSMCs增殖和迁移的机制之一。
Objective： To verify whether homocysteine （Hcy） could induce the dedifferentiation of vascularsmooth muscle cells （VSMCs）. Methods： The primary culture and identification of rat VSMCs was conducted using VSMCs in passage 4-7 for the following experiments. The VSMCs were divided into 4 groups： control group, Hcy （100 μmol/L） modulation group, Hcy （500 μmol/L） modulation group, Hcy （1 000 μmol/L） modulation group. MTT were used to investigate the proliferation of VSMCs. Transwell chambers and wound healing were employed to test the migratory ability of VSMCs. ICC were used to detect the VSMCs＇ morphology structure. Western blotting was used to investigate the expressions of SM-actin, SM-MHC, Calponin, OPN in VSMCs of every group. Results： Compared with control group, the proliferation and migration ability of VSMCs were significantly increased in the Hcy modulation group （P〈0.01）. The expression of SM-actin had no significant dif- ference between each group. The expression of SM-MCH and Calponin increased and OPN decreased in the Hcy group compared with control group （P〈0.01）. This effects of Hcy were positively correlated with its concentra- tions. Conclusion： Hcy can induce the dedifferentiation of VSMCs, this maybe the mechanism by which Hcy increases the proliferation and migration ability of VSMCs.
出处
《温州医科大学学报》
CAS
2016年第1期33-38,共6页
Journal of Wenzhou Medical University
基金
绍兴市科技局科技计划项目(2013B70072)
关键词
同型半胱氨酸
血管平滑肌细胞
平滑肌细胞表型转化
homocysteine
vascular smooth muscle cell
smooth muscle cell phenotype transformation
